Anti-CACNA1A antibody (ab32642)
Key features and details
- Rabbit polyclonal to CACNA1A
- Suitable for: WB, IHC-FoFr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CACNA1A antibody
See all CACNA1A primary antibodies -
Description
Rabbit polyclonal to CACNA1A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow
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Immunogen
Synthetic peptide corresponding to Rat CACNA1A aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab32641)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab32642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use at an assay dependent concentration. Predicted molecular weight: 282 kDa. IHC-FoFr 1/100. Target
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Relevance
Cav2.1 is a voltage-sensitive calcium channels (VSCC) which belongs to the calcium channel alpha-1 subunit family. Cav2.1 mediates the entry of calcium ions into excitable cells and is also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. Cav2.1 (isoform alpha-1A) gives rise to P and/or Q-type calcium currents. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 282648 Cow
- Entrez Gene: 773 Human
- Entrez Gene: 12286 Mouse
- Entrez Gene: 100009265 Rabbit
- Entrez Gene: 25398 Rat
- Omim: 601011 Human
- SwissProt: O00555 Human
- SwissProt: P97445 Mouse
see all -
Alternative names
- APCA antibody
- BI antibody
- Brain calcium channel 1 antibody
see all
Images
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Western blot - Anti-CACNA1A antibody (ab32642)All lanes : Anti-CACNA1A antibody (ab32642) at 1 µg/ml
Lane 1 : E16 Rat Embryo Brain - Tissue Lysate
Lane 2 : E16 Mouse Embryo Brain - Tissue Lysate
Lane 3 : E18 Rat Embryo Brain - Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 282 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab32642 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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Western blot - Anti-CACNA1A antibody (ab32642)Anti-CACNA1A antibody (ab32642) at 1 µg/ml + Cerebellum Rat Tissue Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 282 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-CACNA1A antibody (ab32642)This image is courtesy of Sophie Pezet, Laboratoire de Neurobiologie, Paris, FranceImmunofluorescent staining for CACNA1A using Rabbit polyclonal to CACNA1A (ab32642)in rat Purkinje (arrows) and granular (arrow heads) cells, of the rat cerebellum. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in fre floating IHC. Primary antibody ab32642 was incubated overnight at 1/3000 at room temperature. Secondary antibody Alexa fluor 488 1/3000 was incubated for 2 hours at room temperature.
References (3)
ab32642 has been referenced in 3 publications.
- Corona C et al. Activating Transcription Factor 4 (ATF4) Regulates Neuronal Activity by Controlling GABABR Trafficking. J Neurosci 38:6102-6113 (2018). PubMed: 29875265
- Grauel MK et al. RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses. Proc Natl Acad Sci U S A 113:11615-11620 (2016). PubMed: 27671655
- Vares G et al. Gene silencing of Tead3 abrogates radiation-induced adaptive response in cultured mouse limb bud cells. J Radiat Res (Tokyo) 52:39-46 (2011). WB ; Mouse . PubMed: 21293071
Images
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Western blot - Anti-CACNA1A antibody (ab32642)All lanes : Anti-CACNA1A antibody (ab32642) at 1 µg/ml
Lane 1 : E16 Rat Embryo Brain - Tissue Lysate
Lane 2 : E16 Mouse Embryo Brain - Tissue Lysate
Lane 3 : E18 Rat Embryo Brain - Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 282 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab32642 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
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Western blot - Anti-CACNA1A antibody (ab32642)Anti-CACNA1A antibody (ab32642) at 1 µg/ml + Cerebellum Rat Tissue Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 282 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.
Abcam recommends using milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-CACNA1A antibody (ab32642) This image is courtesy of Sophie Pezet, Laboratoire de Neurobiologie, Paris, FranceImmunofluorescent staining for CACNA1A using Rabbit polyclonal to CACNA1A (ab32642)in rat Purkinje (arrows) and granular (arrow heads) cells, of the rat cerebellum. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in fre floating IHC. Primary antibody ab32642 was incubated overnight at 1/3000 at room temperature. Secondary antibody Alexa fluor 488 1/3000 was incubated for 2 hours at room temperature.
