Anti-SNF2H antibody [3.25(2)] (ab33747)
![Anti-SNF2H antibody [3.25(2)] (ab33747)](https://www.abcam.com/ps/products/33/ab33747/Images/ab33747-47175-anti-snf2h-antibody-3252-immunofluorescence.jpg "Anti-SNF2H antibody [3.25(2)] (ab33747)")
Key features and details
- Mouse monoclonal [3.25(2)] to SNF2H
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SNF2H antibody [3.25(2)]
See all SNF2H primary antibodies -
Description
Mouse monoclonal [3.25(2)] to SNF2H -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Fusion protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following Human whole cell lysates: HeLa, Jurkat, HepG2, MCF7 This antibody gave a negative signal in the following Mouse whole cell lysate: NIH3T3
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General notes
We can conjugate this antibody to FITC for you (please see ab150233 for details).
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3.25(2) -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SNF2H antibody [3.25(2)] (ab33747)ICC/IF image of ab33747 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33747, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10min) HEK293 cells, HepG2 cells at 5ug/ml and in 100% Methanol fixed (5 min) HeLa cells, HEK293 cells, HepG2 cells, and MCF-7 cells at 5µg/ml.
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![Western blot - Anti-SNF2H antibody [3.25(2)] (ab33747)](https://www.abcam.com/ps/products/33/ab33747/Images/ab33747-47174-anti-snf2h-antibody-3252-western-blot.jpg)
Western blot - Anti-SNF2H antibody [3.25(2)] (ab33747)All lanes : Anti-SNF2H antibody [3.25(2)] (ab33747) at 10 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 122 kDa
Observed band size: 122 kDa
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![Flow Cytometry - Anti-SNF2H antibody [3.25(2)] (ab33747)](https://www.abcam.com/ps/products/33/ab33747/Images/ab33747-47173-anti-snf2h-antibody-3252-flow-cytometry.jpg)
Flow Cytometry - Anti-SNF2H antibody [3.25(2)] (ab33747)Overlay histogram showing HeLa cells stained with ab33747 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33747, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
