Anti-Collagen II antibody (ab34712)
Key features and details
- Rabbit polyclonal to Collagen II
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Collagen II antibody
See all Collagen II primary antibodies -
Description
Rabbit polyclonal to Collagen II -
Host species
Rabbit -
Specificity
This antibody reacts with Type II collagens and shows negligible (less than 1%) cross reactivity with Type I, III, IV, V or VI collagens. Nonspecific cross reaction with other human serum proteins or non collagen extracellular matrix proteins is negligible. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human Collagen II.
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Positive control
- IHC-P: Human bronchiolar cartilage; Human Mesenchymal stem cells; Human HEMC-SS spheroids.
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General notes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.435% Sodium chloride, 0.146% EDTA, 4.7625% Sodium borate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross adsorption against other collagens, human serum proteins and non collagen extracellular matrix proteins to remove any unwanted specificities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (ab34712)
ab34712 (1:400, 45 min RT) showed moderate staining in FFPE human bronchiolar cartilage. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (ab34712) Sonomoto K et al. Spontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold. PLoS One 11:e0153231 (2016). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Formalin-fixed, paraffin-embedded human Mesenchymal stem cells stained for collagen II using ab34712 at 1/100 dilution i immunohistochemical analysis.
Healthy donor-derived MSCs were injected into the center of nano-fiber plugs and cultured in MSCGM for the specified period. The samples were assessed immunohistochemically for the expression of type-II collagen on Days 7 and 28. Scale bars, 50 μm.
