Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SNF2H antibody - ChIP Grade (ab3749)

Key features and details

  • Rabbit polyclonal to SNF2H - ChIP Grade
  • Suitable for: IP, ICC/IF, ChIP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-SNF2H antibody - ChIP Grade
    See all SNF2H primary antibodies

  • Description

    Rabbit polyclonal to SNF2H - ChIP Grade

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, ChIP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP (1)
Use at an assay dependent concentration. PubMed: 16917504
ICC/IF (3)
Use at an assay dependent concentration.
ChIP (1)
Use 2.5µl for 106 cells.
WB (4)
1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 122 kDa).
Notes
IP
Use at an assay dependent concentration. PubMed: 16917504
ICC/IF
Use at an assay dependent concentration.
ChIP
Use 2.5µl for 106 cells.
WB
1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 122 kDa).

Target

  • Function

    Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 SANT domains.

  • Developmental stage

    Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • 4933427E24Rik antibody
      • D030040M08Rik antibody
      • D330027N15Rik antibody
      • EC 3.6.1.- antibody
      • hISWI antibody
      • hSNF2H antibody
      • ISWI antibody
      • MommeD4 antibody
      • SMARCA5 antibody
      • SMCA5_HUMAN antibody
      • Snf2h antibody
      • Sucrose nonfermenting like 5 antibody
      • Sucrose nonfermenting protein 2 homolog antibody
      • Sucrose nonfermenting, yeast, homolog of antibody
      • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 antibody
      • WCRF135 antibody
      see all

    Images

    • Western blot - Anti-SNF2H antibody - ChIP Grade (ab3749)
      Western blot - Anti-SNF2H antibody - ChIP Grade (ab3749)

      Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.

      Lane 1: HeLa lysate (20ug)
      Lane 2: Mouse 3T3 lysate (20ug)
      Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
      Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug
       
      Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)

      ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immunising peptide ab5160.

      Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.

      Lane 1: HeLa lysate (20ug)
      Lane 2: Mouse 3T3 lysate (20ug)
      Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
      Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug

      Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)

      ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immun
    • ChIP - Anti-SNF2H antibody - ChIP Grade (ab3749)
      ChIP - Anti-SNF2H antibody - ChIP Grade (ab3749)
       

      ab3749 works in ChIP, as shown by the detection of an increase in the recruitment of SNF2H to the estrogen-responsive pS2 promoter.

        

      Sonicated Chromatin prepared from untreated or 17beta-estradiol (E) treated MCF7 cells was subjected to the ChIP procedure with ab3749 antibody specific to SNF2H. The immunoprecipitated chromatin was analysed in the proximal region of the estro

    References (43)

    Publishing research using ab3749? Please let us know so that we can cite the reference in this datasheet.

    ab3749 has been referenced in 43 publications.

    • Zhong Y  et al. Hierarchical regulation of the resting and activated T cell epigenome by major transcription factor families. Nat Immunol 23:122-134 (2022). PubMed: 34937932
    • Wang Y  et al. LSD1 is required for euchromatic origin firing and replication timing. Signal Transduct Target Ther 7:102 (2022). PubMed: 35414135
    • Abeywickrama-Samarakoon N  et al. Hepatitis Delta Virus histone mimicry drives the recruitment of chromatin remodelers for viral RNA replication. Nat Commun 11:419 (2020). PubMed: 31964889
    • Sabatella M  et al. ERCC1-XPF targeting to psoralen-DNA crosslinks depends on XPA and FANCD2. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31392348
    • Salvador Moreno N  et al. The nuclear structural protein NuMA is a negative regulator of 53BP1 in DNA double-strand break repair. Nucleic Acids Res 47:2703-2715 (2019). PubMed: 30812030
    View all Publications for this product

    Images

    • Western blot - Anti-SNF2H antibody - ChIP Grade (ab3749)
      Western blot - Anti-SNF2H antibody - ChIP Grade (ab3749)

      Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.

      Lane 1: HeLa lysate (20ug)
      Lane 2: Mouse 3T3 lysate (20ug)
      Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
      Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug
       
      Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)

      ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immunising peptide ab5160.

      Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.

      Lane 1: HeLa lysate (20ug)
      Lane 2: Mouse 3T3 lysate (20ug)
      Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
      Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug

      Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)

      ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immun
    • ChIP - Anti-SNF2H antibody - ChIP Grade (ab3749)
      ChIP - Anti-SNF2H antibody - ChIP Grade (ab3749)
       

      ab3749 works in ChIP, as shown by the detection of an increase in the recruitment of SNF2H to the estrogen-responsive pS2 promoter.

        

      Sonicated Chromatin prepared from untreated or 17beta-estradiol (E) treated MCF7 cells was subjected to the ChIP procedure with ab3749 antibody specific to SNF2H. The immunoprecipitated chromatin was analysed in the proximal region of the estro

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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