Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SMARCC1/BAF155 antibody [EPR12389] (ab172636)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12389] to SMARCC1/BAF155
Suitable for: WB, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-SMARCC1/BAF155 antibody [EPR12389]
See all SMARCC1/BAF155 primary antibodies
Description

Rabbit monoclonal [EPR12389] to SMARCC1/BAF155
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human SMARCC1/BAF155 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q92922
Positive control
- NTera2/D1, HeLa, HEK-293 and Jurkat whole cell lysate (ab7899). HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Previously labelled as SMARCC1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12389
Isotype

IgG
Research areas

Western blot - Anti-SMARCC1/BAF155 antibody [EPR12389] (ab172636)

All lanes : Anti-SMARCC1/BAF155 antibody [EPR12389] (ab172636) at 1/5000 dilution

Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : SMARCC1 knockout HEK-293 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 123 kDa
Observed band size: 123 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab172636 observed at 123 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab172636 was shown to specifically react with SMARCC1 in wild-type HEK-293 cells as signal was lost in SMARCC1 knockout cells. Wild-type and SMARCC1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab172636 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody [EPR12389] (ab172636)

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling SMARCC1/BAF155 with ab172636 at a 1/100 dilution.
Western blot - Anti-SMARCC1/BAF155 antibody [EPR12389] (ab172636)

All lanes : Anti-SMARCC1/BAF155 antibody [EPR12389] (ab172636) at 1/1000 dilution

Lane 1 : NTera2/D1 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 123 kDa
Anti-SMARCC1/BAF155 antibody [EPR12389] (ab172636)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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