Alexa Fluor® 647 Anti-SMARCC1/BAF155 antibody [EPR12395] (ab237173)
![Alexa Fluor® 647 Anti-SMARCC1/BAF155 antibody [EPR12395] (ab237173)](https://www.abcam.com/ps/products/237/ab237173/Images/ab237173-2-antiSMARCC1ab237173Flow-CytometryHumanJurkat-Cells.jpg "Alexa Fluor® 647 Anti-SMARCC1/BAF155 antibody [EPR12395] (ab237173)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 647 Rabbit monoclonal [EPR12395] to SMARCC1/BAF155
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Overview
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Product name
Alexa Fluor® 647 Anti-SMARCC1/BAF155 antibody [EPR12395]
See all SMARCC1/BAF155 primary antibodies -
Description
Alexa Fluor® 647 Rabbit monoclonal [EPR12395] to SMARCC1/BAF155 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12395 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Alexa Fluor® 647 Anti-SMARCC1/BAF155 antibody [EPR12395] (ab237173)
Overlay histogram showing Jurkat cells stained with ab237173 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal Goat serum to block non-specific protein-protein interactions followed by the antibody (ab237173) (1x 106 in 100 µl at 0.08 µg/ml (1/6250 dilution)) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SMARCC1/BAF155 antibody [EPR12395] (ab237173)](https://www.abcam.com/ps/products/237/ab237173/Images/ab237173-1-anti-smarcc1-baf155-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-SMARCC1/BAF155 antibody [EPR12395] (ab237173)ab237173 staining SMARCC1 / BAF155 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237173 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).
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![Alexa Fluor® 647 Anti-SMARCC1/BAF155 antibody [EPR12395] (ab237173)](https://www.abcam.com/ps/products/237/ab237173/Images/ab237173-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 647 Anti-SMARCC1/BAF155 antibody [EPR12395] (ab237173)
