Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12395] to SMARCC1/BAF155 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ChIP, WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free
    See all SMARCC1/BAF155 primary antibodies

  • Description

    Rabbit monoclonal [EPR12395] to SMARCC1/BAF155 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ChIP, WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: ICC/IF: HeLa cell lysates. ICC/IF: HeLa cells.

  • General notes

    ab232354 is the carrier-free version of ab172638.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)
    Immunocytochemistry/ Immunofluorescence - Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SMARCC2 with Purified ab172638 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172638).

  • Immunoprecipitation - Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)
    Immunoprecipitation - Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)

    ab172638 (purified) at 1:30 dilution (2μg) immunoprecipitating SMARCC1/BAF155 in Jurkat whole cell lysate.

    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, 10μg
    Lane 2 (+): ab172638 & Jurkat whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab172638 in Jurkat whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST."

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172638).

  • Flow Cytometry - Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)
    Flow Cytometry - Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling SMARCC1/BAF155 with purified ab172638 at 1:30 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172638).

  • ChIP - Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)
    ChIP - Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)
    Chromatin was prepared from MDA-MB-231 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab172638 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci).
    Primers and probes are located in the first kb of the transcribed region.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172638).
  • Immunoprecipitation - Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)
    Immunoprecipitation - Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)

    Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate using unpurified ab172638 at a 1/10 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172638).

  • Flow Cytometry - Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)
    Flow Cytometry - Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)

    Flow cytometric analysis of permeabilized Jurkat cells using unpurified ab172638 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172638).

  • Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)
    Anti-SMARCC1/BAF155 antibody [EPR12395] - BSA and Azide free (ab232354)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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