Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SmarcAL1 antibody [EPR23912-44] - BSA and Azide free (ab277529)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23912-44] to SmarcAL1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SmarcAL1 antibody [EPR23912-44] - BSA and Azide free
    See all SmarcAL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23912-44] to SmarcAL1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ChIP,ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293T, NIH/3T3, C6 and PC-12 whole cell lysates. Flow Cyt: HeLa and NIH/3T3 cells.

  • General notes

    ab277529 is the carrier-free version of ab259972. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277529 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23912-44

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SmarcAL1 antibody [EPR23912-44] - BSA and Azide free (ab277529)
    Western blot - Anti-SmarcAL1 antibody [EPR23912-44] - BSA and Azide free (ab277529)
    All lanes : Anti-SmarcAL1 antibody [EPR23912-44] (ab259972) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 106 kDa
    Observed band size: 105 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab259972, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lysates were made freshly and used in WB test immediately to minimize protein degradation.

    Exposure times: Lanes 1: 92 seconds; Lanes 2-3: 48 seconds; Lanes 4: 26 seconds.

     

  • Flow Cytometry - Anti-SmarcAL1 antibody [EPR23912-44] - BSA and Azide free (ab277529)
    Flow Cytometry - Anti-SmarcAL1 antibody [EPR23912-44] - BSA and Azide free (ab277529)

    This data was developed using ab259972, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling SmarcAL1 with ab259972 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-SmarcAL1 antibody [EPR23912-44] - BSA and Azide free (ab277529)
    Western blot - Anti-SmarcAL1 antibody [EPR23912-44] - BSA and Azide free (ab277529)
    Anti-SmarcAL1 antibody [EPR23912-44] (ab259972) at 1/1000 dilution + C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/2000 dilution

    Predicted band size: 106 kDa
    Observed band size: 105 kDa why is the actual band size different from the predicted?



    This data was developed using ab259972, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Flow Cytometry - Anti-SmarcAL1 antibody [EPR23912-44] - BSA and Azide free (ab277529)
    Flow Cytometry - Anti-SmarcAL1 antibody [EPR23912-44] - BSA and Azide free (ab277529)

    This data was developed using ab259972, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling SmarcAL1 with ab259972 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Anti-SmarcAL1 antibody [EPR23912-44] - BSA and Azide free (ab277529)
    Anti-SmarcAL1 antibody [EPR23912-44] - BSA and Azide free (ab277529)

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