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Epigenetics and Nuclear Signaling Chromatin Remodeling Swi / Snf

Anti-SNF5/SMARCB1 antibody (ab72289)

Price and availability

284 784 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-SNF5/SMARCB1 antibody (ab72289)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to SNF5/SMARCB1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SNF5/SMARCB1 antibody
    See all SNF5/SMARCB1 primary antibodies
  • Description

    Rabbit polyclonal to SNF5/SMARCB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human SNF5/SMARCB1.
    Database link: NP_003064.2

  • Positive control

    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.
  • General notes

     This product was previously labelled as SNF5

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Swi / Snf
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Mediator Complex

Images

  • Western blot - Anti-SNF5/SMARCB1 antibody (ab72289)
    Western blot - Anti-SNF5/SMARCB1 antibody (ab72289)
    All lanes : Anti-SNF5/SMARCB1 antibody (ab72289) at 0.4 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 44 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 140 kDa, 200 kDa, 28 kDa, 38 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody (ab72289)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody (ab72289)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SNF5/SMARCB1 with ab72289 at 1/1000 (1µg/ml). Detection: DAB.

  • Immunoprecipitation - Anti-SNF5/SMARCB1 antibody (ab72289)
    Immunoprecipitation - Anti-SNF5/SMARCB1 antibody (ab72289)

    Detection of Human SNF5/SMARCB1 by Immunoprecipitation from HeLa whole cell lysate, (1 mg for IP, 20% of IP loaded), using ab72289 at 3µg/mg lysate for IP and at 1µg/ml for subsequent WB.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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