Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20189] to SNF5/SMARCB1
Suitable for: IHC-P, Flow Cyt, IP, ICC/IF, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-SNF5/SMARCB1 antibody [EPR20189]
See all SNF5/SMARCB1 primary antibodies
Description

Rabbit monoclonal [EPR20189] to SNF5/SMARCB1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, Jurkat, PC-12 and NIH-3T3 whole cell lysates; Human fetal brain, fetal heart and fetal spleen lysates; Mouse and rat brain and spleen lysates. IHC-P: Human, mouse and rat kidney tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
General notes
This product was previously labelled as SNF5

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20189
Isotype

IgG
Research areas

Western blot - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

All lanes : Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : G-401 (Human rhabdoid tumor kidney epithelial cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Negative control: G-401 (PMID: 19789351).
Western blot - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

All lanes : Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519) at 1/1000 dilution

Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
Lane 4 : Human fetal brain lysate at 10 µg
Lane 5 : Human fetal heart lysate at 10 µg
Lane 6 : Human fetal spleen lysate at 10 µg
Lane 7 : Mouse brain lysate at 10 µg
Lane 8 : Mouse spleen lysate at 10 µg
Lane 9 : Rat brain lysate at 10 µg
Lane 10 : Rat spleen lysate at 10 µg

Secondary
Lanes 1-3 & 7-10 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 4-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 44 kDa
Observed band size: 44 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times : Lane 1-3: 2 seconds; Lanes 4-5/7: 15 seconds; Lane 6/8-9: 4 seconds; Lane 10: 1 minute.
Immunoprecipitation - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

SNF5/SMARCB1 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab222519 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222519 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: HeLa whole cell lysate 10 μg (input).
Lane 2: ab222519 IP in HeLa whole cell lysate,
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222519 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling SNF5/SMARCB1 with ab222519 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Nuclear staining in human kidney is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling SNF5/SMARCB1 with ab222519 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Nuclear staining in mouse kidney is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling SNF5/SMARCB1 with ab222519 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), Ready to use. Nuclear staining in rat kidney is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SNF5/SMARCB1 with ab222519 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining in HeLa cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Negative control: G-401 cell line (PMID:19789351).
Flow Cytometry - Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeablized G-401 (Human rhabdoid tumor kidney epithelial cell line, Left) and HeLa (Human epithelial cell line from cervix adenocarcinoma, Right) cells labeling SNF5/SMARCB1 with ab222519 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control: G-401 (PMID:19789351)
Anti-SNF5/SMARCB1 antibody [EPR20189] (ab222519)

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