Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)
![Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)](https://www.abcam.com/ps/products/20/ab20194/Images/ab20194-212421-anti-hsv1-icp8-major-dna-binding-protein-antibody-11e2-immunofluorescence.jpg "Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)")
Key features and details
- Mouse monoclonal [11E2] to HSV1 ICP8 Major DNA binding protein
- Suitable for: IP, WB, ICC/IF
- Isotype: IgG1
Overview
-
Product name
Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2]
See all HSV1 ICP8 Major DNA binding protein primary antibodies -
Description
Mouse monoclonal [11E2] to HSV1 ICP8 Major DNA binding protein -
Host species
Mouse -
Tested applications
Suitable for: IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Full length native protein (purified from U-35-VERO cells) (HSV).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
11E2 -
Isotype
IgG1 -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194) This image is courtesy of an anonymous Abreview
ab20194 staining HSV1 ICP8 Major DNA binding protein in Human U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
-
![Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)](https://www.abcam.com/ps/products/20/ab20194/Images/ab20194-29522-anti-hsv1-icp8-major-dna-binding-protein-antibody-11e2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194) Image from Ohta A et al., Virol J. 2011 Jul 26;8:365. Fig 5.; doi:10.1186/1743-422X-8-365; 26 July 2011, Virology Journal 2011, 8:365Immunofluorescence analysis of cells infected with HSV1, staining HSV1 ICP8 Major DNA binding protein (purple) with ab20194, 7 (left) or 17 (right) hours after infection.
Cells were permeabilized in 0.1% Triton X-100 in PBS for 5 min at room temperature before blocking with blocking buffer (4% goat serum, 1% BSA in PBS-Tween [0.05%]) for 30 min at room temperature. Samples were incubated with primary antibody (1/1000) and a fluorescence conjugated anti-mouse IgG was used to detect staining. -
![Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194)](https://www.abcam.com/ps/products/20/ab20194/Images/ab20194-29521-anti-hsv1-icp8-major-dna-binding-protein-antibody-11e2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (ab20194) Image from Alazard-Dany N et al., PLoS Pathog. 2009 Mar;5(3):e1000340. Epub 2009 Mar 13. Fig 5.; doi:10.1371/journal.ppat.1000340; March 13, 2009, PLoS Pathog 5(3): e1000340.Immunofluorescence analysis of HeLa cells transfected with pTF3 and pRF, staining HSV1 ICP8 Major DNA binding protein with ab20194.
Cells were fixed in paraformaldehyde for 10 min and then permeabilized with 0.5% Triton X-100 for 30 min. The cells were blocked with 4% BSA + 0.2% Tween for 30 min before incubation for 1 hour at RT with primary antibody (1/200 diluted in PBS-T). An AlexaFluor®488-conjugated donkey anti-mouse IgG (1/2000) was used as the secondary antibody.
