Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (ab35026)
![Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (ab35026)](https://www.abcam.com/ps/products/35/ab35026/Images/ab35026-308625-anti-cd81-antibody-1d6-low-endotoxin-flow-cytometry.jpg "Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (ab35026)")
Key features and details
- Mouse monoclonal [1D6] to CD81 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD81 antibody [1D6] - Low endotoxin, Azide free
See all CD81 primary antibodies -
Description
Mouse monoclonal [1D6] to CD81 - Low endotoxin, Azide free -
Host species
Mouse -
Specificity
ab35026 recognises CD81, a 26kD cell surface antigen and a member of the tetraspanin family. -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD81. Aggregated OCI-LY8 cells.
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Positive control
- Flow Cyt: HAP1-WT cells.
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General notes
Ab35026 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti proliferative effects. Endotoxin Level:
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
This antibody was purified from tissue culture supernatant. -
Primary antibody notes
Ab35026 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti proliferative effects. Endotoxin Level:Clonality
MonoclonalClone number
1D6Myeloma
P3x63-Ag8.653Isotype
IgG1Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab35026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes Flow Cyt 1/5 - 1/10.Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Notes Flow Cyt
1/5 - 1/10.Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.Application notesIs unsuitable for WB.Target
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Function
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV. -
Tissue specificity
Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines. -
Involvement in disease
Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsNot glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 975 Human
- Omim: 186845 Human
- SwissProt: P60033 Human
- Unigene: 54457 Human
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Alternative names
- 26 kDa cell surface protein TAPA 1 antibody
- 26 kDa cell surface protein TAPA-1 antibody
- 26 kDa cell surface protein TAPA1 antibody
see all
Images
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Flow Cytometry - Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (ab35026)
Overlay histogram showing HAP1 wildtype (green line) and HAP1-CD81 knockout cells (red line) stained with ab35026. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab35026, 0.1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.
A mouse IgG1 isotype control antibody (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CD81 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
Datasheets and documents
References (4)
ab35026 has been referenced in 4 publications.
- Baruah P et al. TLR9 Mediated Tumor-Stroma Interactions in Human Papilloma Virus (HPV)-Positive Head and Neck Squamous Cell Carcinoma Up-Regulate PD-L1 and PD-L2. Front Immunol 10:1644 (2019). PubMed: 31379843
- Wu B et al. Exosomes isolated from CAPS1-overexpressing colorectal cancer cells promote cell migration. Oncol Rep 42:2528-2536 (2019). PubMed: 31638236
- Ngalame NNO et al. Arsenic Alters Exosome Quantity and Cargo to Mediate Stem Cell Recruitment Into a Cancer Stem Cell-Like Phenotype. Toxicol Sci 165:40-49 (2018). PubMed: 30169766
- Drummond HA et al. Renal inflammation and elevated blood pressure in a mouse model of reduced {beta}-ENaC. Am J Physiol Renal Physiol 301:F443-9 (2011). WB ; Mouse . PubMed: 21543417
Images
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Flow Cytometry - Anti-CD81 antibody [1D6] - Low endotoxin, Azide free (ab35026)
Overlay histogram showing HAP1 wildtype (green line) and HAP1-CD81 knockout cells (red line) stained with ab35026. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab35026, 0.1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.
A mouse IgG1 isotype control antibody (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CD81 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
