Antibodies, Proteins, Kits and Reagents for Life Science

368 544 ₸ Product size

100 µl 368 544 ₸ 10 µl 150 768 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4244] to CD81
Suitable for: WB
Reacts with: Mouse, Rat, Human

Product name

Anti-CD81 antibody [EPR4244]
See all CD81 primary antibodies
Description

Rabbit monoclonal [EPR4244] to CD81
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Ramos, JAR, U87-MG, IM-9, peripheral blood mononuclear cell (PBMC), C6, PC-12, and RAW264.7 cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4244
Isotype

IgG
Research areas

Western blot - Anti-CD81 antibody [EPR4244] (ab109201)

All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/700 dilution (unpurified)

Lane 1 : Ramos cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : Raw264.7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 26 kDa
Observed band size: 20 kDa
why is the actual band size different from the predicted?
Western blot - Anti-CD81 antibody [EPR4244] (ab109201)

All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/2000 dilution (purified)

Lane 1 : Ramos cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : Raw264.7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 26 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Western blot - Anti-CD81 antibody [EPR4244] (ab109201)

All lanes : Anti-CD81 antibody [EPR4244] (ab109201) at 1/1000 dilution (unpurified)

Lane 1 : Ramos cell lysate
Lane 2 : JAR cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : IM-9 cell lysate
Lane 5 : Peripheral blood mononuclear cell (PBMC) cell lysate
Lane 6 : C6 cell lysate
Lane 7 : PC-12 cell lysate
Lane 8 : RAW264.7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

Predicted band size: 26 kDa
Anti-CD81 antibody [EPR4244] (ab109201)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com