Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD4 antibody [EPR20122] - BSA and Azide free (ab251480)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20122] to CD4 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD4 antibody [EPR20122] - BSA and Azide free
    See all CD4 primary antibodies

  • Description

    Rabbit monoclonal [EPR20122] to CD4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251480 is the carrier-free version of ab207755. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251480 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-CD4 antibody [EPR20122] - BSA and Azide free (ab251480)
    Flow Cytometry - Anti-CD4 antibody [EPR20122] - BSA and Azide free (ab251480)
    This data was developed using ab207755, the same antibody clone in a different buffer formulation.Flow cytometric analysis of Mouse thymocytes labeling CD4 with ab207755 at 1/60 dilution (red), compared with ab172730 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
  • Western blot - Anti-CD4 antibody [EPR20122] - BSA and Azide free (ab251480)
    Western blot - Anti-CD4 antibody [EPR20122] - BSA and Azide free (ab251480)
    Anti-CD4 antibody [EPR20122] (ab207755) at 1/5000 dilution + Mouse thymus tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa


    Exposure time: 5 seconds


    This data was developed using ab207755, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-CD4 antibody [EPR20122] - BSA and Azide free (ab251480)
    Immunoprecipitation - Anti-CD4 antibody [EPR20122] - BSA and Azide free (ab251480)
    This data was developed using ab207755, the same antibody clone in a different buffer formulation.CD4 was immunoprecipitated from 0.35 mg of Mouse thymus lysate with ab207755 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab207755 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1: Mouse thymus lysate, 10 μg (Input). Lane 2: ab207755 IP in Mouse thymus lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207755 in Mouse thymus lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds.
  • Anti-CD4 antibody [EPR20122] - BSA and Azide free (ab251480)
    Anti-CD4 antibody [EPR20122] - BSA and Azide free (ab251480)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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