Anti-CD38 antibody [90] - BSA and Azide free (ab239595)
![Anti-CD38 antibody [90] - BSA and Azide free (ab239595)](https://www.abcam.com/ps/products/239/ab239595/Images/ab239595-344270-AntiCD38-antibody-90--BSA-and-Azide-free-flow-cytometry-mouse-splenocytes.jpg "Anti-CD38 antibody [90] - BSA and Azide free (ab239595)")
Key features and details
- Rat monoclonal [90] to CD38 - BSA and Azide free
- Suitable for: Flow Cyt, IHC-Fr
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-CD38 antibody [90] - BSA and Azide free
See all CD38 primary antibodies -
Description
Rat monoclonal [90] to CD38 - BSA and Azide free -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseIHC-Fr Mouse
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Immunogen
Tissue, cells or virus corresponding to Mouse CD38. Mouse pre-B cells.
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Positive control
- Flow Cyt: C57/BL6 splenocytes. IHC-Fr: Mouse Spleen
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General notes
ab239595 is a PBS-only buffer format of ab61400. Please refer to ab61400 for recommended dilutions, protocols, and image data.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
In mouse CD38 is expressed by follicular B cells, is down regulated on germinal centre B cells, and not expressed by mature plasma cells. This pattern of expression contrasts strikingly with that seen in the human.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
90 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-CD38 antibody [90] - BSA and Azide free (ab239595)
C57BL/6 mouse splenocytes stained with ab61400 (right) or Rat IgG2aΚ (monoclonal) (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10 µg/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab61400) or Rat IgG2a Isotype (1x106 in 100µl at 1 µg/ml) (left) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min at 4°. The cells were simultaneously stained with CD19 APC conjugated antibody.
Acquisition of >50,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab61400).
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![Immunohistochemistry (Frozen sections) - Anti-CD38 antibody [90] - BSA and Azide free (ab239595)](https://www.abcam.com/ps/products/239/ab239595/Images/ab239595-365862-anticd3890msspleenihcfr.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD38 antibody [90] - BSA and Azide free (ab239595)This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab61400).
IHC image of CD38 staining in a section of frozen normal mouse Spleen.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab61400 at 1µg/ml. The section was then incubated with ab150165 (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preabsorbed, (Shown in green) 1/1000) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue). The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
