Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD21 antibody [SP186] - C-terminal (ab227662)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP186] to CD21 - C-terminal
  • Suitable for: IHC-P, WB, Flow Cyt
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-CD21 antibody [SP186] - C-terminal
    See all CD21 primary antibodies

  • Description

    Rabbit monoclonal [SP186] to CD21 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Daudi cell lysate. Flow Cyt: Raji and 2.4G2 cells. IHC-P: Human tonsil, reactive lymph node, spleen and colon tissues, rat spleen

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP186

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD21 with ab227662 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227662 for 10 mins at room temperature.

  • Flow Cytometry - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Flow Cytometry - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Flow Cytometry analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD21 with purified ab227662 at 1:20 dilution (5.3 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
  • Western blot - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Western blot - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Anti-CD21 antibody [SP186] - C-terminal (ab227662) at 1/400 dilution + Daudi (Human Burkitt's lymphoma cell line) cell lysate

    Predicted band size: 113 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling CD21 with ab227662 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227662 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD21 with ab227662 at 1/100 dilution (1.06 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227662 for 10 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Flow Cytometry - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Flow Cytometry analysis of 2.4G2 (rat B cell lymphoma B lymphocyte) cells labeling CD21 with purified ab227662 at 1:200 dilution (0.53 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
  • Flow Cytometry - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Flow Cytometry - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

    Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cell line labeling CD21 with ab227662 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

    Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

    Formalin-fixed, paraffin-embedded human spleen tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP186] - C-terminal (ab227662)

    Formalin-fixed, paraffin-embedded human colon tissue stained for CD21 using ab227662 at 1/100 dilution in immunohistochemical analysis.

  • Anti-CD21 antibody [SP186] - C-terminal (ab227662)
    Anti-CD21 antibody [SP186] - C-terminal (ab227662)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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