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Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3093] to CD21 - Low endotoxin, Azide free
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free
    See all CD21 primary antibodies

  • Description

    Rabbit monoclonal [EP3093] to CD21 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt (Intra)

  • Species reactivity

    Reacts with: Human
    Predicted to work with: MouseDoes not react with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Daudi cell lysate, Raji cell lysate and Tonsil tissue IP: Daudi whole cell lysate.

  • General notes

    ab193554 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP3093

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
    Flow Cytometry - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554) This image is courtesy of an anonymous Abreview.
    ab75985 staining CD21 in Cynomolgus Monkey whole blood by Flow Cytometry. Red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide, fixed in paraformaldehyde and incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
    Gating Strategy: Live Lymphocytes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

  • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
    Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

    ab75985 staining CD21 antibody in Raji (human Burkitt's lymphoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

    DAPI was used as a nuclear counterstain and the negative control was PBS only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

    ab75985 showing positive staining in Normal tonsil tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

  • Immunoprecipitation - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
    Immunoprecipitation - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

    Purified ab75985 at 1/100 dilution (2µg) immunoprecipitating CD21 in Daudi whole cell lysate.
    Lane 1 (input): Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate 10µg
    Lane 2 (+): ab75985 + Daudi whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75985 in Daudi whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 155 kDa
    The band at around 15kDa could be cleavage form. (PMID: 9647256)
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

    ab75985 showing negative staining in Normal liver tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

    ab75985 showing negative staining in Normal kidney tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

    ab75985 showing negative staining in Normal breast tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

    This IHC data was generated using the same anti-CD21 antibody clone, EP3093, in a different buffer formulation (cat# ab75985).

    ab75985 showing positive staining in Normal tonsil tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
    Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

    This ICC/IF data was generated using the same anti-CD21 antibody clone, EP3093, in a different buffer formulation (cat# ab75985).

    ab75985 staining CD21 antibody in Raji (human Burkitt's lymphoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

    DAPI was used as a nuclear counterstain and the negative control was PBS only.

  • Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)
    Anti-CD21 antibody [EP3093] - Low endotoxin, Azide free (ab193554)

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