Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP199] to CD21 - BSA and Azide free
Suitable for: Flow Cyt, IHC-P, WB
Reacts with: Human

Product name

Anti-CD21 antibody [SP199] - BSA and Azide free
See all CD21 primary antibodies
Description

Rabbit monoclonal [SP199] to CD21 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, IHC-P, WBmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Daudi cell lysate.IHC-P: Human tonsil and reactive lymph node tissue.Flow cytometry: Raji cells.
General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Ab240988 is the carrier-free version of ab227668. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240988 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP199
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD21 with ab227668 at 1/100 dilution (1.02 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227668 for 10 mins at room temperature.

This image was generated using ab227668, the same clone, but with a different buffer formulation.
Flow Cytometry - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)

Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cell line labeling CD21 with ab227668 at 1/100 (green) compared with a rabbit isotype control (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227668).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD21 with ab227668 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227668).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)

Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for CD21 with ab227668 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227668).
Anti-CD21 antibody [SP199] - BSA and Azide free (ab240988)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com