Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Immunology Adaptive Immunity T Cells CD

Anti-CD21 antibody [EP3093] (ab75985)

Price and availability

358 492 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-CD21 antibody [EP3093] (ab75985)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3093] to CD21
  • Suitable for: ICC/IF, WB, IP, IHC-P, Flow Cyt
  • Reacts with: Human

You may also be interested in

Product image
PerCP Anti-CD6/T12 antibody [MAE-1C10] (ab90898)
Product image
PE/Cy5.5® Anti-CD69 antibody [FN50] (ab272252)
Product image
Recombinant Human DC-SIGN protein (ab131780)
Product image
redFluor™ 710 Anti-CD45RA antibody [HI100] (ab218648)

Overview

  • Product name

    Anti-CD21 antibody [EP3093]
    See all CD21 primary antibodies
  • Description

    Rabbit monoclonal [EP3093] to CD21
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CD21 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human normal tonsil tissue. ICC: Raji cells. IP: Daudi cell lysate. Flow Cyt: Cynomolgus monkey whole blood. WB: Daudi and Raji cell lysate.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP3093
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] (ab75985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] (ab75985)

    ab75985 showing positive staining in human normal tonsil tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] (ab75985)
    Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] (ab75985)

    ab75985 staining CD21 antibody in Raji (human Burkitt's lymphoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

    DAPI was used as a nuclear counterstain and the negative control was PBS only.

  • Immunoprecipitation - Anti-CD21 antibody [EP3093] (ab75985)
    Immunoprecipitation - Anti-CD21 antibody [EP3093] (ab75985)

    Purified ab75985 at 1/100 dilution (2µg) immunoprecipitating CD21 in Daudi whole cell lysate.
    Lane 1 (input): Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate 10µg
    Lane 2 (+): ab75985 + Daudi whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75985 in Daudi whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 155 kDa
    The band at around 15kDa could be cleavage form. (PMID: 9647256)

  • Western blot - Anti-CD21 antibody [EP3093] (ab75985)
    Western blot - Anti-CD21 antibody [EP3093] (ab75985)
    All lanes : Anti-CD21 antibody [EP3093] (ab75985) at 1/2000 dilution

    Lane 1 : Daudi (Human Burkitt's lymphoma cell line) cell lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 113 kDa
    Observed band size: 155 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-CD21 antibody [EP3093] (ab75985)
    Flow Cytometry - Anti-CD21 antibody [EP3093] (ab75985) This image is courtesy of an anonymous Abreview

    ab75985 staining CD21 in Cynomolgus monkey whole blood by Flow Cytometry. Red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide, fixed in paraformaldehyde and incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
    Gating Strategy: Live Lymphocytes.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] (ab75985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] (ab75985)

    ab75985 showing negative staining in human normal liver tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] (ab75985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] (ab75985)

    ab75985 showing negative staining in human normal kidney tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] (ab75985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] (ab75985)

    ab75985 showing negative staining in human normal brain tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] (ab75985)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] (ab75985)

    ab75985 showing negative staining in human normal breast tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-CD21 antibody [EP3093] (ab75985)
    Anti-CD21 antibody [EP3093] (ab75985)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD21 antibody [EP3093] (ab75985)

  •  
  • Anti-CD21 antibody [1F8], prediluted (ab7290)

    Applications:

  •  
  • Product image

    Anti-CD21 antibody - C-terminal (ab203299)

    Applications: Flow Cyt, IHC-P

  •  
  • Product image

    PE/Cy5® Anti-CD21 antibody [LT21] (ab201303)

    Applications: Flow Cyt

  •  
  • Product image

    Anti-CD21 antibody [Bu32] (ab54253)

    Applications: Flow Cyt

  •  
  • Product image

    HRP Anti-CD21 antibody [EP3093] (ab202354)

    Applications: IHC-P, WB

  •  
  • Product image

    PE/Cy7® Anti-CD21 antibody [LT21] (ab234211)

    Applications: Flow Cyt

  •  
  • Product image

    Anti-CD21 antibody [CR2/1953] (ab237981)

    Applications: IHC-P

  •  
  • Product image

    Anti-CD21 antibody [Q22-S] - C-terminal (ab190511)

    Applications: IHC-P

  •  
  • Product image

    Anti-CD21 antibody [SP199] (ab227668)

    Applications: Flow Cyt, IHC-P, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-SerpinB8 antibody (ab235602)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.