Human IL-12B Matched Antibody Pair Kit (ab222270)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 15.6 pg/ml - 1000 pg/ml
Overview
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Product name
Human IL-12B Matched Antibody Pair Kit
See all IL-12B kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
1.6 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human IL-12B Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human IL-12B.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab220656. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 10 x 96 tests 5 x 96 tests Human IL-12B Capture Antibody 3201039 2 x 50µg 1 x 50µg Human IL-12B Detector Antibody 2 x 12.5µg 1 x 12.5µg Human IL-12B Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
Involvement in disease
Mendelian susceptibility to mycobacterial disease
Psoriasis 11 -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Post-translational
modificationsKnown to be C-mannosylated in the recombinant protein; it is not yet known for sure if the wild-type protein is also modified. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CLMF
- CLMF p40
- CLMF2
see all -
Database links
- Entrez Gene: 3593 Human
- Omim: 161561 Human
- SwissProt: P29460 Human
- Unigene: 674 Human
Images
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Human IL-12B standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human IL-12B Matched Antibody Pair Kit (ab222270)To learn more about the advantages of recombinant antibodies see here.
