Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸ 10 µl 134 016 ₸

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Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6966] to SNF5/SMARCB1
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-SNF5/SMARCB1 antibody [EPR6966]
See all SNF5/SMARCB1 primary antibodies
Description

Rabbit monoclonal [EPR6966] to SNF5/SMARCB1
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, Jurkat, K562, Daudi and HEK293T cell lysates.
General notes
This product was previously labelled as SNF5

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6966
Isotype

IgG
Research areas

Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)

All lanes : Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : SMARCB1 knockout HEK293T cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 44 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab126734 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa.

ab126734 Anti-SNF5/SMARCB1 antibody [EPR6966] was shown to specifically react with SNF5/SMARCB1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267269 (knockout cell lysate ab257688) was used. Wild-type and SNF5/SMARCB1 knockout samples were subjected to SDS-PAGE. ab126734 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)

All lanes : Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

Developed using the ECL technique.

Predicted band size: 44 kDa
Western blot - Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734) This image is courtesy of an anonymous Abreview

Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734) at 1/2000 dilution + Zebrafish whole mount purified protein at 15 µg

Secondary
Undiluted HRP-conjugated goat anti-rabbit IgG polyclonal

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 15 kDa (possible non-specific binding), 28 kDa (possible non-specific binding)

Exposure time: 15 seconds
See Abreview
Anti-SNF5/SMARCB1 antibody [EPR6966] (ab126734)

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