Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸ 10 µl 113 243 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SNF5/SMARCB1 antibody [EPR12014-77] (ab192864)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12014-77] to SNF5/SMARCB1
Suitable for: IHC-P, Flow Cyt, IP, ICC/IF, WB
Knockout validated
Reacts with: Human

Product name

Anti-SNF5/SMARCB1 antibody [EPR12014-77]
See all SNF5/SMARCB1 primary antibodies
Description

Rabbit monoclonal [EPR12014-77] to SNF5/SMARCB1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Daudi, HEK293T, HeLa, Jurkat and K562 cell lysate. IHC-P: Human kidney tissue; Human squamous cell carcinoma of cervix tissue. ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.
General notes
This product was previously labelled as SNF5

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12014-77
Isotype

IgG
Research areas

Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014-77] (ab192864)

All lanes : Anti-SNF5/SMARCB1 antibody [EPR12014-77] (ab192864) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : SMARCB1 knockout HEK293T cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 44 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab192864 observed at 50 kDa. Red - loading control ab8245 observed at 36 kDa.

ab192864 Anti-SNF5/SMARCB1 antibody [EPR12014-77] was shown to specifically react with SNF5/SMARCB1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267269 (knockout cell lysate ab257688) was used. Wild-type and SNF5/SMARCB1 knockout samples were subjected to SDS-PAGE. ab192864 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR12014-77] (ab192864)

Immunohistochemical analysis of paraffin embedded Human kidney tissue sections labeling SNF5/SMARCB1 using ab192864 at a 1/1000 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF5/SMARCB1 antibody [EPR12014-77] (ab192864)

Immunohistochemical analysis of paraffin embedded Human squamous cell carcinoma of cervix tissue sections labeling SNF5/SMARCB1 using ab192864 at a 1/1000 dilution. A predilutedHRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-SNF5/SMARCB1 antibody [EPR12014-77] (ab192864)

Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling SNF5/SMARCB1 using ab192864 at a 1/500 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.
Flow Cytometry - Anti-SNF5/SMARCB1 antibody [EPR12014-77] (ab192864)

Flow cytometric analysis of Jurkat cells labeling SNF5/SMARCB1 using ab192864 at a 1/110 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary antibody at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (black). Unlabeled/control cells without incubation with primary and secondary antibody (blue). Cells were fixed in 2% paraformaldehyde.
Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014-77] (ab192864)

All lanes : Anti-SNF5/SMARCB1 antibody [EPR12014-77] (ab192864) at 1/50000 dilution

Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Immunoprecipitation - Anti-SNF5/SMARCB1 antibody [EPR12014-77] (ab192864)

Western blot analysis of K562 cell lysate immunoprecipitated using ab192864 at a 1/30 dilution (lane 1).
Lane 2: PBS instead of K562 lysate
Secondary antibody was anti-rabbit IgG (HRP) specific to the non-reduced form of IgG at a 1/1500 dilution.
Anti-SNF5/SMARCB1 antibody [EPR12014-77] (ab192864)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com