Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12014] to SNF5/SMARCB1 - BSA and Azide free
  • Suitable for: IP, WB

Overview

  • Product name

    Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free
    See all SNF5/SMARCB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR12014] to SNF5/SMARCB1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250544 is the carrier-free version of ab181976 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250544 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as SNF5

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544)
    Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544)
    All lanes : Anti-SNF5/SMARCB1 antibody [EPR12014] (ab181976) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa



    This data was developed using ab181976, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544)
    Western blot - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544)
    All lanes : Anti-SNF5/SMARCB1 antibody [EPR12014] (ab181976) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 44 kDa



    This data was developed using ab181976, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544)
    Immunoprecipitation - Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544)
    This data was developed using ab181976, the same antibody clone in a different buffer formulation.Western blot analysis of HeLa cell lysate immunoprecipitated with ab181976 at 1/50 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG used at 1/1500 dilution.
  • Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544)
    Anti-SNF5/SMARCB1 antibody [EPR12014] - BSA and Azide free (ab250544)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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