Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TTF2 antibody [EPR12756] (ab181116)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12756] to TTF2
Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
Reacts with: Human

Product name

Anti-TTF2 antibody [EPR12756]
See all TTF2 primary antibodies
Description

Rabbit monoclonal [EPR12756] to TTF2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Daudi and HeLa cell line lysates; Jurkat cells; Human adenocarcinoma of endometrium tissue; Human pancreas tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12756
Isotype

IgG
Research areas

Western blot - Anti-TTF2 antibody [EPR12756] (ab181116)

All lanes : Anti-TTF2 antibody [EPR12756] (ab181116) at 1/5000 dilution

Lane 1 : Daudi cell line lysate
Lane 2 : HeLa cell line lysate
Lane 3 : Jurkat cell line lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 130 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] (ab181116)

Immunohistochemical analysis of paraffin embedded Human adenocarcinoma of endometrium tissue labeling TTF2 with ab181116 at 1/250. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] (ab181116)

Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling TTF2 with ab181116 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TTF2 antibody [EPR12756] (ab181116)

Immunofluorescent analysis of acetone-fixed Jurkat cells labeling TTF2 with ab181116 at 1/100 (green) dilution. Goat anti rabbit IgG (Alexa Fluor® 488) was used for secondary antibody staining at a dilution of 1/200. Counter stain: Dapi (blue)
Flow Cytometry - Anti-TTF2 antibody [EPR12756] (ab181116)

Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling TTF2 with ab181116 at 1/60 dilution, followed by Goat anti rabbit IgG (FITC) at 1/150 dilution (pink). Rabbit monoclonal IgG was used as Isotype control (green).
Anti-TTF2 antibody [EPR12756] (ab181116)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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