Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TTF2 antibody [EPR12756] (ab181116)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12756] to TTF2
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TTF2 antibody [EPR12756]
    See all TTF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12756] to TTF2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Daudi and HeLa cell line lysates; Jurkat cells; Human adenocarcinoma of endometrium tissue; Human pancreas tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TTF2 antibody [EPR12756] (ab181116)
    Western blot - Anti-TTF2 antibody [EPR12756] (ab181116)
    All lanes : Anti-TTF2 antibody [EPR12756] (ab181116) at 1/5000 dilution

    Lane 1 : Daudi cell line lysate
    Lane 2 : HeLa cell line lysate
    Lane 3 : Jurkat cell line lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 130 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] (ab181116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] (ab181116)

    Immunohistochemical analysis of paraffin embedded Human adenocarcinoma of endometrium tissue labeling TTF2 with ab181116 at 1/250. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] (ab181116)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF2 antibody [EPR12756] (ab181116)

    Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling TTF2 with ab181116 at 1/250 dilution. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Counter stain: Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TTF2 antibody [EPR12756] (ab181116)
    Immunocytochemistry/ Immunofluorescence - Anti-TTF2 antibody [EPR12756] (ab181116)

    Immunofluorescent analysis of acetone-fixed Jurkat cells labeling TTF2 with ab181116 at 1/100 (green) dilution. Goat anti rabbit IgG (Alexa Fluor® 488) was used for secondary antibody staining at a dilution of 1/200. Counter stain: Dapi (blue)

  • Flow Cytometry - Anti-TTF2 antibody [EPR12756] (ab181116)
    Flow Cytometry - Anti-TTF2 antibody [EPR12756] (ab181116)

    Flow cytometric analysis of  2% paraformaldehyde-fixed Jurkat cells labeling TTF2 with ab181116 at 1/60 dilution, followed by Goat anti rabbit IgG (FITC) at 1/150 dilution (pink). Rabbit monoclonal IgG was used as Isotype control (green).

  • Anti-TTF2 antibody [EPR12756] (ab181116)
    Anti-TTF2 antibody [EPR12756] (ab181116)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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