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Epigenetics and Nuclear Signaling Chromatin Remodeling Swi / Snf

Anti-BAF170 antibody (ab264170)

Anti-BAF170 antibody (ab264170)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to BAF170
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-BAF170 antibody
    See all BAF170 primary antibodies
  • Description

    Rabbit polyclonal to BAF170
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human BAF170 aa 1164-1214 (C terminal). The exact sequence is proprietary. NP_003066.2
    Database link: Q8TAQ2

  • Positive control

    • IHC-P: Human skin basal cell carcinoma. WB: HeLa and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Swi / Snf
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Images

  • Western blot - Anti-BAF170 antibody (ab264170)
    Western blot - Anti-BAF170 antibody (ab264170)
    All lanes : Anti-BAF170 antibody (ab264170) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : NIH/3T3 whole cell lysate at 50 µg

    Secondary
    All lanes : Goat anti-Rabbit Light Chain HRP Conjugate

    Predicted band size: 133 kDa


    Exposure time: 1 second


    4-8% SDS-PAGE

  • Immunoprecipitation - Anti-BAF170 antibody (ab264170)
    Immunoprecipitation - Anti-BAF170 antibody (ab264170)

    BAF170 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264170 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264170 at 1 µg/ml.

    Lane 1: ab264170 IP in HeLa whole cell lysate.
    Lane 2: Control IgG.

    Exposure time: 1 sec.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF170 antibody (ab264170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF170 antibody (ab264170)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin basal cell carcinoma tissue labeling BAF170 with ab264170 at 1/200 dilution. Detection: DAB.

    Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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