Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23103-44] to SMARCA2 / BRM - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free
    See all SMARCA2 / BRM primary antibodies

  • Description

    Rabbit monoclonal [EPR23103-44] to SMARCA2 / BRM - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HeLa and Daudi whole cell lysates. IHC-P: Human renal cell carcinoma, mouse cerebrum and rat kidney tissue. HeLa and Daudi cell lysates. ICC/IF: HeLa and Neuro-2a cells. Flow Cyt (intra): HeLa cells.

  • General notes

    ab269872 is the carrier-free version of ab240648.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling SMARCA2/BRM with ab240648 at 1/2000 dilution (0.23 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat kidney. The section was incubated with ab240648 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240648).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SMARCA2/BRM with ab240648 at 1/2000 dilution (0.23 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse cerebrum. The section was incubated with ab240648 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240648).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue labeling SMARCA2/BRM with ab240648 at 1/2000 dilution (0.23 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human renal cell carcinoma. The section was incubated with ab240648 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240648).

  • Flow Cytometry - Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)
    Flow Cytometry - Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling SMARCA2/BRM with ab240648 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240648).

  • Immunocytochemistry/ Immunofluorescence - Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)
    Immunocytochemistry/ Immunofluorescence - Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling SMARCA2/BRM with ab240648 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear and weak cytoplasmic staining in Neuro-2a cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240648).

  • Immunocytochemistry/ Immunofluorescence - Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)
    Immunocytochemistry/ Immunofluorescence - Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling SMARCA2/BRM with ab240648 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear and weak cytoplasmic staining in HeLa cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab240648).

  • Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)
    Anti-SMARCA2 / BRM antibody [EPR23103-44] - BSA and Azide free (ab269872)

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