Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20860-251] to SMARCD2
Suitable for: WB, IHC-P, Flow Cyt, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-SMARCD2 antibody [EPR20860-251]
See all SMARCD2 primary antibodies
Description

Rabbit monoclonal [EPR20860-251] to SMARCD2
Host species

Rabbit
Specificity

IHC is recommended only for mouse and rat.

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Mouse Rat
IP	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Neuro-2a cells transfected with scrambled siRNA control, whole cell lysate; mouse P70 thymus, white adipose, brown adipose and liver lysate; Beta-TC-6 whole cell lysate; Rat thymus lysate; Jurkat and HeLa whole cell lysate. IHC-P: Mouse colon tissue; Rat testis tissue. Flow cyt: HeLa cells. IP: HeLa whole cell lysate; Mouse P70 thymus lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20860-251
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling SMARCD2 with ab220164 at 1/200 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat testis (PMID: 20148946, 28303890, 25622893). The section was incubated with ab220164 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunoprecipitation - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

SMARCD2 was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab220164 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220164 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab220164 IP in HeLa whole cell lysate .
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220164 in HeLa whole cell lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 40 seconds.
Flow Cytometry - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling SMARCD2 with ab220164 at 1/600 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling SMARCD2 with ab220164 at 1/200 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse colon (PMID: 20148946, 28303890, 25622893). The section was incubated with ab220164 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunoprecipitation - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

SMARCD2 was immunoprecipitated from 0.35 mg mouse P70 thymus lysate with ab220164 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220164 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.

Lane 1: Mouse P70 thymus lysate 10 µg (Input).
Lane 2: ab220164 IP in mouse P70 thymus lysate .
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220164 in mouse P70 thymus lysate.

Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 seconds.
Western blot - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

All lanes : Anti-SMARCD2 antibody [EPR20860-251] (ab220164) at 1/1000 dilution

Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Rat thymus lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 59 kDa
Observed band size: 59 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 59 secs; Lane 2: 81 secs; Lane 3: 26 secs.
Western blot - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

All lanes : Anti-SMARCD2 antibody [EPR20860-251] (ab220164) at 1/1000 dilution

Lane 1 : Mouse P70 liver lysate
Lane 2 : Mouse P70 thymus lysate
Lane 3 : Mouse P70 white adipose lysate
Lane 4 : Mouse P70 brown adipose lysate
Lane 5 : Beta-TC-6 (mouse pancreas insulinoma beta cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 59 kDa
Observed band size: 59 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

All lanes : Anti-SMARCD2 antibody [EPR20860-251] (ab220164) at 1/1000 dilution

Lane 1 : Neuro-2a (mouse neuroblastoma cell line) transfected with scrambled siRNA control whole cell lysate
Lane 2 : SMARCD2 knockdown Neuro-2a whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 59 kDa
Observed band size: 59 kDa

Exposure time: 3 minutes

Lysates were kindly provided by Dr Zhuoxian Meng.

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-SMARCD2 antibody [EPR20860-251] (ab220164)

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