Antibodies, Proteins, Kits and Reagents for Life Science

264 681 ₸ Product size

100 µl 264 681 ₸

Order now and get it on Tuesday March 09, 2021

Anti-eIF3g antibody [EPR16147] (ab192601)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16147] to eIF3g
Suitable for: WB, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-eIF3g antibody [EPR16147]
See all eIF3g primary antibodies
Description

Rabbit monoclonal [EPR16147] to eIF3g
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- 293T, MOLT4, K562, mouse Raw 264.7, mouse NIH 3T3 and rat PC12 cell lysates. HeLa cells.
General notes
This product was previously labelled as EIF3S4

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16147
Isotype

IgG
Research areas

Western blot - Anti-eIF3g antibody [EPR16147] (ab192601)

All lanes : Anti-eIF3g antibody [EPR16147] (ab192601) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : K562 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 36 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?
Western blot - Anti-eIF3g antibody [EPR16147] (ab192601)

All lanes : Anti-eIF3g antibody [EPR16147] (ab192601) at 1/1000 dilution

Lane 1 : mouse Raw 264.7 cell lysate
Lane 2 : rat PC12 cell lysate
Lane 3 : mouse NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 36 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-eIF3g antibody [EPR16147] (ab192601)

Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling eIF3g with ab192601 at a 1/100 dilution ( 7.0 μg/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/400 dilution. In blue DAPI staining.
Immunoprecipitation - Anti-eIF3g antibody [EPR16147] (ab192601)

Western blot analysis on immunoprecipitation from 1) K562 cell lysate and 2) PBS, labeling eIF3g using ab192601 at 1/40 dilution and HRP-conjugated anti-rabbit IgG specific to the non-reduced form of rabbit IgG at a 1/1500 dilution.
Anti-eIF3g antibody [EPR16147] (ab192601)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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