Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16146] to eIF3g - N-terminal
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-eIF3g antibody [EPR16146] - N-terminal
    See all eIF3g primary antibodies

  • Description

    Rabbit monoclonal [EPR16146] to eIF3g - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, Molt-4, HepG2, K562, PC-12 and NIH/3T3 cell lysate. Human colonic carcinoma tissue. Rat skeletal muscle tissue. HepG2 cells. 293T cells.

  • General notes

     

     

     This product was previously labelled as EIF3S4

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)
    Western blot - Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)
    All lanes : Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Molt-4 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)
    Western blot - Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)
    All lanes : Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422) at 1/1000 dilution

    Lane 1 : PC-12 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)

    Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue sections labeling eIF3g using ab191422 at a 1/50 dilution. A ready to use HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)

    Immunohistochemical analysis of paraffin embedded rat skeletal muscle tissue sections labeling eIF3g using ab191422 at a 1/50 dilution. A ready to use HRP polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HepG2 cells labeling eIF3g using ab191422 at a 1/150 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/200 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100. The two negative controls: Primary ab concentration (anti-EIF3G), Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/400 dilution.

  • Flow Cytometry - Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)
    Flow Cytometry - Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)

    Flow Cytometry analysis of 2% paraformaldehyde fixed 293T cells labeling eIF3g using ab191422 at a 1/80 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green).

  • Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)
    Anti-eIF3g antibody [EPR16146] - N-terminal (ab191422)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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