Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566)
![Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566)](https://www.abcam.com/ps/products/184/ab184566/Images/ab184566-217809-anti-ttc10-antibody-epr14850-n-terminal-western-blot.jpg "Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14850] to IFT88 - N-terminal
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Overview
-
Product name
Anti-IFT88 antibody [EPR14850] - N-terminal
See all IFT88 primary antibodies -
Description
Rabbit monoclonal [EPR14850] to IFT88 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Human fetal kidney, HepG2 and Jurkat whole cell lysate (ab7899); HepG2 cells.
-
General notes
This product was previously labelled as TTC10
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14850 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566)All lanes : Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566) at 1/50000 dilution
Lane 1 : 293 cell lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 94 kDa
-
![Flow Cytometry - Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566)](https://www.abcam.com/ps/products/184/ab184566/Images/ab184566-217811-anti-ttc10-antibody-epr14850-n-terminal-flow-cytometry.jpg)
Flow Cytometry - Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566)Flow cytometric analysis of HepG2 cells (2% paraformaldehyde-fixed) labeling IFT88 with ab184566 at 1/260 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
-
![Western blot - Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566)](https://www.abcam.com/ps/products/184/ab184566/Images/ab184566-217810-anti-ttc10-antibody-epr14850-n-terminal-western-blot.jpg)
Western blot - Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566)All lanes : Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 94 kDa
-
![Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566)](https://www.abcam.com/ps/products/184/ab184566/Images/ab184566-4-benefits-of-recombinant-antibodies.png)
Anti-IFT88 antibody [EPR14850] - N-terminal (ab184566)
