Anti-IFT88 antibody - C-terminal (ab240910)
Key features and details
- Rabbit polyclonal to IFT88 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IFT88 antibody - C-terminal
See all IFT88 primary antibodies -
Description
Rabbit polyclonal to IFT88 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human IFT88 aa 674-686 (C terminal). NP_001305420.1
Database link: Q13099 -
Positive control
- WB: A549 cell lysate. ICC/IF: SK-N-BE cells.
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General notes
This product was previously labelled as TTC10
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IFT88 antibody - C-terminal (ab240910)All lanes : Anti-IFT88 antibody - C-terminal (ab240910) at 1/1000 dilution (1 hour at RT)
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293Trap cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit HRP for 60 minutes at RT at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 6 minutesBlock: 5% Skimmed milk in 1X TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-IFT88 antibody - C-terminal (ab240910)Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (human neuroblastoma cell line) cells labeling IFT88 using ab240910 at 1/100 dilution for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 minutes at RT.
Counterstains: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab240910 (D) Composite.
