Anti-IFT88 antibody (ab42497)
Key features and details
- Goat polyclonal to IFT88
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IFT88 antibody
See all IFT88 primary antibodies -
Description
Goat polyclonal to IFT88 -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2). -
Tested applications
Suitable for: ICC/IF, WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Dog
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Immunogen
Synthetic peptide corresponding to Human IFT88 aa 804-816 (C terminal).
Sequence:C-KKRIDEDDFADEE
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Positive control
- WB: A549 cell lysate and Mouse testis tissue lysate; ICC/IF: HepG2 cells; Flow Cyt: HepG2 cells.
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General notes
This product was previously labelled as TTC10
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IFT88 antibody (ab42497)All lanes : Anti-IFT88 antibody (ab42497) at 3 µg/ml
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Mouse testis tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 94 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-IFT88 antibody (ab42497)Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells labeling IFT88 with ab42497. Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor® 488 conjugated secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Flow Cytometry - Anti-IFT88 antibody (ab42497)Flow cytometric analysis of paraformaldehyde fixed HepG2 cells labeling IFT88 with ab42497 (blue line). Cells permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488® secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-IFT88 antibody (ab42497) This image is a courtesy of Luke Hofkampab42497 staining IFT88 in human prostate epithelial primary cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton ×100. Samples were incubated with primary antibody (1/100:in DMEM w/ 10% FBS) for 1 hour at 200C. An Alexa Fluor ® 546-conjugated donkey polyclonal to goat IgG was used undiluted as secondary antibody.
