Antibodies, Proteins, Kits and Reagents for Life Science

247 259 ₸ Product size

100 µl 247 259 ₸

Order now and get it on Tuesday March 09, 2021

Anti-MRPS18B antibody [EPR19298] (ab191891)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19298] to MRPS18B
Suitable for: ICC/IF, IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-MRPS18B antibody [EPR19298]
See all MRPS18B primary antibodies
Description

Rabbit monoclonal [EPR19298] to MRPS18B
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal liver, fetal heart and fetal kidney lysates; Mouse heart, kidney and spleen lysates; and Rat kidney and liver lysates. MCF7, HeLa, Jurkat, HepG2, C6, RAW 264.7, NIH/3T3 whole cell lysates. ICC/IF: HeLa and MCF7 cell lines. IP: MCF7 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19298
Isotype

IgG
Research areas

Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891)

All lanes : Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

Exposure time: 3 minutes

5% NFDM/TBST: Blocking and dilution buffer.
Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891)

All lanes : Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

Exposure time: 3 minutes

5% NFDM/TBST: Blocking and dilution buffer.
Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891)

All lanes : Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat kidney lysate
Lane 5 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

Exposure time: 3 minutes

5% NFDM/TBST: Blocking and dilution buffer.
Western blot - Anti-MRPS18B antibody [EPR19298] (ab191891)

All lanes : Anti-MRPS18B antibody [EPR19298] (ab191891) at 1/2000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

Exposure time: 15 seconds

5% NFDM/TBST: Blocking and dilution buffer.
Immunocytochemistry/ Immunofluorescence - Anti-MRPS18B antibody [EPR19298] (ab191891)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling MRPS18B with ab191891 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on HeLa cell line.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
1. ab191891 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
2. ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MRPS18B antibody [EPR19298] (ab191891)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cell line labeling MRPS18B with ab191891 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on MCF7 cell line.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
1. ab191891 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
2. ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
Immunoprecipitation - Anti-MRPS18B antibody [EPR19298] (ab191891)

MRPS18B was immunoprecipitated from 1mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab191891 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab191891 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: MCF7 whole cell lysate, 10ug (Input).
Lane 2: ab191891 IP in MCF7 whole cell lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab191891 in MCF7 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Anti-MRPS18B antibody [EPR19298] (ab191891)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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