Anti-EBP1 antibody (ab35424)
Key features and details
- Rabbit polyclonal to EBP1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-EBP1 antibody
See all EBP1 primary antibodies -
Description
Rabbit polyclonal to EBP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Synthetic peptide corresponding to Human EBP1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab35423) -
Positive control
- Recombinant Human EBP1 protein (ab95350) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Heart (Mouse) Tissue Lysate Testis (Mouse) Tissue Lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab35424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 1 µg/ml. WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Target
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Function
May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). -
Tissue specificity
Expressed in several cell lines tested, including primary and transformed cell lines. -
Sequence similarities
Belongs to the peptidase M24 family. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG. - Information by UniProt
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Database links
- Entrez Gene: 540272 Cow
- Entrez Gene: 5036 Human
- Entrez Gene: 18813 Mouse
- Entrez Gene: 288778 Rat
- Omim: 602145 Human
- SwissProt: Q9UQ80 Human
- SwissProt: P50580 Mouse
- Unigene: 524498 Human
see all -
Alternative names
- 38kDa antibody
- AA672939 antibody
- Cell cycle protein p38 2G4 homolog antibody
see all
Images
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Western blot - Anti-EBP1 antibody (ab35424)Lane 1 : Marker
Lanes 2-3 : Anti-EBP1 antibody (ab35424) at 1 µg/ml
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 :Jurkat whole cell lysate (ab7899) at 20 µg
Secondary
Lanes 2-3 : IRDye 680 conjugated Goat anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44,54 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands.This antibody detects a band corresponding to EBP1 at the predicted molecular weight of 44 kDa. An additional band of ~54 kDa is also detected. We are unsure of the identity of this band, although this may represent a modified form of the protein.
Our other antibody to this target (ab33613), raised against a distinct epitope, also detects the major band at 44 kDa. In contrast to ab35424 however, an additional band at 38 kDa is detected by ab33613. These distinct banding patterns suggest that this protein exists in multiple forms.
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Western blot - Anti-EBP1 antibody (ab35424)All lanes : Anti-EBP1 antibody (ab35424) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Testis (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 55 kDa, 70 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-EBP1 antibody (ab35424)ICC/IF image of ab35424 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab35424, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
References (0)
ab35424 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-EBP1 antibody (ab35424)Lane 1 : Marker
Lanes 2-3 : Anti-EBP1 antibody (ab35424) at 1 µg/ml
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 :Jurkat whole cell lysate (ab7899) at 20 µg
Secondary
Lanes 2-3 : IRDye 680 conjugated Goat anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44,54 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands.This antibody detects a band corresponding to EBP1 at the predicted molecular weight of 44 kDa. An additional band of ~54 kDa is also detected. We are unsure of the identity of this band, although this may represent a modified form of the protein.
Our other antibody to this target (ab33613), raised against a distinct epitope, also detects the major band at 44 kDa. In contrast to ab35424 however, an additional band at 38 kDa is detected by ab33613. These distinct banding patterns suggest that this protein exists in multiple forms.
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Western blot - Anti-EBP1 antibody (ab35424)All lanes : Anti-EBP1 antibody (ab35424) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Testis (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 55 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
-
Immunocytochemistry/ Immunofluorescence - Anti-EBP1 antibody (ab35424)ICC/IF image of ab35424 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab35424, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
