Anti-BRCC45/BRE antibody (ab95984)
Key features and details
- Rabbit polyclonal to BRCC45/BRE
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-BRCC45/BRE antibody
See all BRCC45/BRE primary antibodies -
Description
Rabbit polyclonal to BRCC45/BRE -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Zebrafish -
Immunogen
Recombinant fragment corresponding to Human BRCC45/BRE aa 9-338.
Database link: Q9NXR7 -
Positive control
- WB: HeLa, HEK-293T, A431 whole cell lysate. Rat testis. C8D30 whole cell lysate. IP: HEK-293T cells. ICC/IF: HeLa cells.
-
General notes
This product was previously labelled as BRCC45
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunofluorescence analysis of BRCC45/BRE in paraformaldehyde fixed HeLa, using ab95984 at 1/200 dilution. Lower image merged with DNA probe.
-
All lanes : Anti-BRCC45/BRE antibody (ab95984) at 1/500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : BRCC45/BRE Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 47 kDa
-
BRE was immunoprecipitated from 100 μg of HEK-293T whole cell lysate with ab95984 at 1/1000 dilution. EasyBlot anti-rabbit IgG was used as a secondary reagent. 10% SDS-PAGE.
A: Control with 2.5 μg of preimmune rabbit IgG.
B: ab95984 (2.5 μg) IP of BRE protein. -
Anti-BRCC45/BRE antibody (ab95984) at 1/1000 dilution + C8D30 whole cell lysate at 30 µg
Predicted band size: 47 kDa10 % SDS-PAGE
-
Rat tissue extract at 50 µg
Predicted band size: 47 kDa10 % SDS-PAGE
-
All lanes : Anti-BRCC45/BRE antibody (ab95984) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa10 % SDS-PAGE.