Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11858] to BRCC45/BRE
  • Suitable for: WB, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-BRCC45/BRE antibody [EPR11858]
    See all BRCC45/BRE primary antibodies

  • Description

    Rabbit monoclonal [EPR11858] to BRCC45/BRE

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human BRCC45/BRE aa 1-100. The exact sequence is proprietary.
    Database link: Q9NXR7

  • Positive control

    • WB: HeLa, SH-SY5Y, Daudi and Raji cell lysates; Fetal brain and human placenta tissue lysates. IHC-P: Human lung adenocarcinoma and pancreas tissue. ICC/IF: MCF-7 cells.

  • General notes

     This product was previously labelled as BRCC45

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    All lanes : Anti-BRCC45/BRE antibody [EPR11858] (ab177960) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : BRCC45/BRE knockout HeLa cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 44 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab177960 observed at 44 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab177960 Recombinant Anti-BRE antibody [EPR11858] was shown to specifically react with BRE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264928 (knockout cell lysate ab257861) was used. Wild-type and BRE knockout samples were subjected to SDS-PAGE. ab177960 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

    Immunohistochemical analysis of Human pancreas tissue labeling BRCC45/BRE using ab177960 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Immunocytochemistry/ Immunofluorescence - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

    Immunofluorescence analysis of MCF-7 cells labeling BRCC45/BRE using ab177960 at 1/50 dilution.

  • Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    All lanes : Anti-BRCC45/BRE antibody [EPR11858] (ab177960) at 1/1000 dilution

    Lane 1 : fetal brain lysate
    Lane 2 : Human placenta lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 44 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

    Immunohistochemical analysis of Human lung adenocarcinoma tissue labeling BRCC45/BRE using ab177960 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
    Anti-BRCC45/BRE antibody [EPR11858] (ab177960)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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