Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
![Anti-BRCC45/BRE antibody [EPR11858] (ab177960)](https://www.abcam.com/ps/products/177/ab177960/Images/ab177960-389671-recombinant-anti-brcc45bre-antibody-epr11858-western-blot-human-lysate.jpg "Anti-BRCC45/BRE antibody [EPR11858] (ab177960)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11858] to BRCC45/BRE
- Suitable for: WB, ICC/IF, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-BRCC45/BRE antibody [EPR11858]
See all BRCC45/BRE primary antibodies -
Description
Rabbit monoclonal [EPR11858] to BRCC45/BRE -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human BRCC45/BRE aa 1-100. The exact sequence is proprietary.
Database link: Q9NXR7 -
Positive control
- WB: HeLa, SH-SY5Y, Daudi and Raji cell lysates; Fetal brain and human placenta tissue lysates. IHC-P: Human lung adenocarcinoma and pancreas tissue. ICC/IF: MCF-7 cells.
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General notes
This product was previously labelled as BRCC45
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11858 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)All lanes : Anti-BRCC45/BRE antibody [EPR11858] (ab177960) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BRCC45/BRE knockout HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 44 kDaLanes 1-4: Merged signal (red and green). Green - ab177960 observed at 44 kDa. Red - loading control ab7291 observed at 50 kDa.
ab177960 Recombinant Anti-BRE antibody [EPR11858] was shown to specifically react with BRE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264928 (knockout cell lysate ab257861) was used. Wild-type and BRE knockout samples were subjected to SDS-PAGE. ab177960 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)](https://www.abcam.com/ps/products/177/ab177960/Images/ab177960-206004-anti-brcc45-antibody-epr11858-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)Immunohistochemical analysis of Human pancreas tissue labeling BRCC45/BRE using ab177960 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)](https://www.abcam.com/ps/products/177/ab177960/Images/ab177960-206002-anti-brcc45-antibody-epr11858-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)Immunofluorescence analysis of MCF-7 cells labeling BRCC45/BRE using ab177960 at 1/50 dilution.
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![Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)](https://www.abcam.com/ps/products/177/ab177960/Images/ab177960-206001-anti-brcc45-antibody-epr11858-western-blot.jpg)
Western blot - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)All lanes : Anti-BRCC45/BRE antibody [EPR11858] (ab177960) at 1/1000 dilution
Lane 1 : fetal brain lysate
Lane 2 : Human placenta lysate
Lane 3 : HeLa cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 44 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)](https://www.abcam.com/ps/products/177/ab177960/Images/ab177960-206003-anti-brcc45-antibody-epr11858-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRCC45/BRE antibody [EPR11858] (ab177960)Immunohistochemical analysis of Human lung adenocarcinoma tissue labeling BRCC45/BRE using ab177960 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-BRCC45/BRE antibody [EPR11858] (ab177960)](https://www.abcam.com/ps/products/177/ab177960/Images/ab177960-6-benefits-of-recombinant-antibodies.png)
Anti-BRCC45/BRE antibody [EPR11858] (ab177960)
