Anti-DDAH1 antibody (ab127579)
Key features and details
- Rabbit polyclonal to DDAH1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDAH1 antibody
See all DDAH1 primary antibodies -
Description
Rabbit polyclonal to DDAH1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Macaque monkey, Gorilla, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Human DDAH1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab127966) -
Positive control
- This antibody gave a positive signal in both HEK293 and HepG2 whole cell lysates as well as the following tissue lysates: Human Fetal Liver; Human Liver; Human Kidney; Human Pancreas.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab127579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB 1/250. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa). IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Target
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Function
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. -
Tissue specificity
Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle. -
Sequence similarities
Belongs to the DDAH family. - Information by UniProt
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Database links
- Entrez Gene: 23576 Human
- Entrez Gene: 69219 Mouse
- Entrez Gene: 64157 Rat
- Omim: 604743 Human
- SwissProt: P56965 Cow
- SwissProt: O94760 Human
- SwissProt: Q9CWS0 Mouse
- SwissProt: O08557 Rat
see all -
Alternative names
- DDAH antibody
- DDAH I antibody
- DDAH-1 antibody
see all
Images
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Western blot - Anti-DDAH1 antibody (ab127579)All lanes : Anti-DDAH1 antibody (ab127579) at 1 µg/ml
Lane 1 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Human kidney tissue lysate - total protein (ab30203)
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 6 : Human pancreas tissue lysate - total protein (ab29816)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH1 antibody (ab127579)IHC image of DDAH1 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab127579, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References (1)
ab127579 has been referenced in 1 publication.
- Lin HH et al. DDAH-2 alleviates contrast medium iopromide-induced acute kidney injury through nitric oxide synthase. Clin Sci (Lond) 133:2361-2378 (2019). PubMed: 31763675
Images
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Western blot - Anti-DDAH1 antibody (ab127579)All lanes : Anti-DDAH1 antibody (ab127579) at 1 µg/ml
Lane 1 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : Human kidney tissue lysate - total protein (ab30203)
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 6 : Human pancreas tissue lysate - total protein (ab29816)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDAH1 antibody (ab127579)IHC image of DDAH1 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab127579, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
