Anti-DDX51 antibody (ab72292)
Key features and details
- Rabbit polyclonal to DDX51
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDX51 antibody -
Description
Rabbit polyclonal to DDX51 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide cprresponding to a region between residues 616 and 666 of human DDX51 (NP_778236.1)
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Positive control
- HeLa whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab72292 was affinity purified using an epitope specific to DDX51 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-DDX51 antibody (ab72292) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Additional bands at: 172 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
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Detection of DDX51 by Western Blot of Immunprecipitate.
Lane 1: ab72292, at 1µg/ml, staining DDX51 in HeLa whole cell lysate immunoprecipitated using ab72292 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
Lane 2: Control IgG. Detection: Chemiluminescence with an exposure time of 3 minutes.