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Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free (ab256478)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22908-103] to eRF3/GSPT1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free
    See all eRF3/GSPT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22908-103] to eRF3/GSPT1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His tagged human eRF3a recombinant protein, PC-3, HeLa, RAW264.7 and NIH/3T3 lysates.

  • General notes

    Ab256478 is the carrier-free version of ab234433. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256478 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free (ab256478)
    Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free (ab256478)
    All lanes : Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) at 1/1000 dilution

    Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate,
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution


    This antibody recognizes unidentifiable weak bands below 50kDa

    Exposure time: Lanes 1-2: 3 seconds Lanes 3-4: 37 seconds

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234433).

  • Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free (ab256478)
    Western blot - Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free (ab256478)
    All lanes : Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) at 1/1000 dilution

    Lane 1 : His tagged human eRF3a recombinant protein, 10 ng
    Lane 2 : His tagged human eRF3b recombinant protein, 10ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution


    Exposure time: 15 seconds

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234433).

  • Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free (ab256478)
    Anti-eRF3/GSPT1 antibody [EPR22908-103] - BSA and Azide free (ab256478)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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