Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22908-103] to eRF3/GSPT1
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-eRF3/GSPT1 antibody [EPR22908-103]
See all eRF3/GSPT1 primary antibodies -
Description
Rabbit monoclonal [EPR22908-103] to eRF3/GSPT1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His tagged human eRF3a recombinant protein, PC-3, HeLa, RAW264.7 and NIH/3T3 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22908-103 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) at 1/1000 dilution
Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 80 kDa why is the actual band size different from the predicted?This antibody recognizes unidentifiable weak bands below 50kDa
Exposure time: Lanes 1-2: 3 seconds Lanes 3-4: 37 seconds
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-eRF3/GSPT1 antibody [EPR22908-103] (ab234433) at 1/1000 dilution
Lane 1 : His tagged human eRF3a recombinant protein
Lane 2 : His tagged human eRF3b recombinant protein, 10ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 48 kDa why is the actual band size different from the predicted?Exposure time: 15 seconds
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST
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