Anti-TRMT5 antibody [EPR24139-11] (ab259986)
![Anti-TRMT5 antibody [EPR24139-11] (ab259986)](https://www.abcam.com/ps/products/259/ab259986/Images/ab259986-453242-anti-trmt5-antibody-epr2413911-western-blot-cell.jpg "Anti-TRMT5 antibody [EPR24139-11] (ab259986)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24139-11] to TRMT5
- Suitable for: WB, ICC, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Overview
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Product name
Anti-TRMT5 antibody [EPR24139-11]
See all TRMT5 primary antibodies -
Description
Rabbit monoclonal [EPR24139-11] to TRMT5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseHumanICC MouseHumanWB MouseHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat; A431; MCF7; U-2 OS; NIH/3T3 whole cell lysates. ICC: A431; NIH/3T3 cells. Flow cyt: A431; NIH/3T3 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24139-11 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TRMT5 antibody [EPR24139-11] (ab259986)All lanes : Anti-TRMT5 antibody [EPR24139-11] (ab259986) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 58 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lane 5 of the blot was developed using a higher sensitivity ECL substrate.
Exposure time: Lane 1-4: 3 minutes
Lane 5: 103 seconds
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![Immunocytochemistry - Anti-TRMT5 antibody [EPR24139-11] (ab259986)](https://www.abcam.com/ps/products/259/ab259986/Images/ab259986-1-anti-trmt5-antibody-epr2413911-immunocytochemistry-a431.jpg)
Immunocytochemistry - Anti-TRMT5 antibody [EPR24139-11] (ab259986)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling TRMT5 with ab259986 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weak cytoplasmic staining in A431 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry - Anti-TRMT5 antibody [EPR24139-11] (ab259986)](https://www.abcam.com/ps/products/259/ab259986/Images/ab259986-3-anti-trmt5-antibody-epr2413911-flow-cytometry-a431.jpg)
Flow Cytometry - Anti-TRMT5 antibody [EPR24139-11] (ab259986)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431 (Human epidermoid carcinoma epithelial cell) cells labelling TRMT5 with ab259986 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. -
![Immunocytochemistry - Anti-TRMT5 antibody [EPR24139-11] (ab259986)](https://www.abcam.com/ps/products/259/ab259986/Images/ab259986-2-anti-trmt5-antibody-epr2413911-immunocytochemistry-nih3t3.jpg)
Immunocytochemistry - Anti-TRMT5 antibody [EPR24139-11] (ab259986)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling TRMT5 with ab259986 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weak cytoplasmic staining in NIH/3T3 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry - Anti-TRMT5 antibody [EPR24139-11] (ab259986)](https://www.abcam.com/ps/products/259/ab259986/Images/ab259986-4-anti-trmt5-antibody-epr2413911-flow-cytometry-nih3t3.jpg)
Flow Cytometry - Anti-TRMT5 antibody [EPR24139-11] (ab259986)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling TRMT5 with ab259986 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
