Anti-RPS12 antibody [EPR10964(B)] (ab167428)
![Anti-RPS12 antibody [EPR10964(B)] (ab167428)](https://www.abcam.com/ps/products/167/ab167428/Images/RPS12-Primary-antibodies-ab167428-1.jpg "Anti-RPS12 antibody [EPR10964(B)] (ab167428)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10964(B)] to RPS12
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RPS12 antibody [EPR10964(B)]
See all RPS12 primary antibodies -
Description
Rabbit monoclonal [EPR10964(B)] to RPS12 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Rat
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Immunogen
Synthetic peptide, corresponding to residues in Human RPS12 (Uniprot: P25398).
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Positive control
- C6, RAW264.7, NIH 3T3, HeLa, MCF7, HepG2 and Raji cell lysates; Human colon and kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10964(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RPS12 antibody [EPR10964(B)] (ab167428)All lanes : Anti-RPS12 antibody [EPR10964(B)] (ab167428) at 1/1000 dilution
Lane 1 : Rat C6 cell lysate
Lane 2 : Mouse RAW 264.7 cell lysate
Lane 3 : Mouse NIH 3T3 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : MCF7 cell lysate
Lane 6 : HepG2 cell lysate
Lane 7 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 15 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS12 antibody [EPR10964(B)] (ab167428)](https://www.abcam.com/ps/products/167/ab167428/Images/ab167428-7-YCAR10964-Ribosomal-Protein-S12-uterus.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS12 antibody [EPR10964(B)] (ab167428)ab167428 showing +ve staining in Human normal uterus.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS12 antibody [EPR10964(B)] (ab167428)](https://www.abcam.com/ps/products/167/ab167428/Images/ab167428-6-YCAR10964-Ribosomal-Protein-S12-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS12 antibody [EPR10964(B)] (ab167428)ab167428 showing +ve staining in Human normal tonsil.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS12 antibody [EPR10964(B)] (ab167428)](https://www.abcam.com/ps/products/167/ab167428/Images/ab167428-5-YCAR10964-Ribosomal-Protein-S12-stomach.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS12 antibody [EPR10964(B)] (ab167428)ab167428 showing +ve staining in Human normal stomach.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS12 antibody [EPR10964(B)] (ab167428)](https://www.abcam.com/ps/products/167/ab167428/Images/ab167428-4-YCAR10964-Ribosomal-Protein-S12-brain.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS12 antibody [EPR10964(B)] (ab167428)ab167428 showing +ve staining in Human normal brain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS12 antibody [EPR10964(B)] (ab167428)](https://www.abcam.com/ps/products/167/ab167428/Images/RPS12-Primary-antibodies-ab167428-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS12 antibody [EPR10964(B)] (ab167428)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue, labeling RPS12 using ab167428 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS12 antibody [EPR10964(B)] (ab167428)](https://www.abcam.com/ps/products/167/ab167428/Images/RPS12-Primary-antibodies-ab167428-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS12 antibody [EPR10964(B)] (ab167428)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling RPS12 using ab167428 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-RPS12 antibody [EPR10964(B)] (ab167428)](https://www.abcam.com/ps/products/167/ab167428/Images/ab167428-8-benefits-of-recombinant-antibodies.png)
Anti-RPS12 antibody [EPR10964(B)] (ab167428)
