Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸ 10 µl 107 212 ₸

Order now and get it on Tuesday March 09, 2021

Anti-RPS12 antibody [EPR10963] (ab175219)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10963] to RPS12
Suitable for: Flow Cyt, WB, IP, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-RPS12 antibody [EPR10963]
See all RPS12 primary antibodies
Description

Rabbit monoclonal [EPR10963] to RPS12
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RPS12 aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P25398
Positive control
- HeLa, MCF-7, HepG2, Raji, C6, Raw264.7 and NIH-3T3 cell lysates; MCF7 cells; Hela cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10963
Isotype

IgG
Research areas

Western blot - Anti-RPS12 antibody [EPR10963] (ab175219)

All lanes : Anti-RPS12 antibody [EPR10963] (ab175219) at 1/1000 dilution

Lane 1 : Hela cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Raji cell lysate
Lane 5 : C6 cell lysate
Lane 6 : Raw 264.7 cell lysate
Lane 7 : NIH-3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 15 kDa
Immunoprecipitation - Anti-RPS12 antibody [EPR10963] (ab175219)

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate using ab175219 at 1/10 dilution.
Flow Cytometry - Anti-RPS12 antibody [EPR10963] (ab175219)

Flow cytometric analysis of permeabilized HeLa cells labeling RPS12 with ab175219 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Immunocytochemistry/ Immunofluorescence - Anti-RPS12 antibody [EPR10963] (ab175219)

Immunofluorescent staining of MCF7 cells labeling RPS12 with ab175219 at 1/50 dilution (green). DAPI nuclear staining (blue).
Anti-RPS12 antibody [EPR10963] (ab175219)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com