Anti-NUDT5 antibody [EPR7735] (ab129163)
![Anti-NUDT5 antibody [EPR7735] (ab129163)](https://www.abcam.com/ps/products/129/ab129163/Images/ab129163-397643-anti-nudt5-antibody-epr7735-western-blot-human-knockout-lysate.jpg "Anti-NUDT5 antibody [EPR7735] (ab129163)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7735] to NUDT5
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-NUDT5 antibody [EPR7735]
See all NUDT5 primary antibodies -
Description
Rabbit monoclonal [EPR7735] to NUDT5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human NUDT5 aa 150-250. The exact sequence is proprietary.
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Positive control
- WB: HeLa, 293T, HepG2 and U937 cell lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.48 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7735 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)All lanes : Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : NUDT5 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab129163 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.
ab129163 Anti-NUDT5 antibody [EPR7735] was shown to specifically react with NUDT5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266242 (knockout cell lysate ab258087) was used. Wild-type and NUDT5 knockout samples were subjected to SDS-PAGE. ab129163 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)](https://www.abcam.com/ps/products/129/ab129163/Images/ab129163-143163-anti-nudt5-antibody-epr7735-western-blot.jpg)
Western blot - Anti-NUDT5 antibody [EPR7735] (ab129163)All lanes : Anti-NUDT5 antibody [EPR7735] (ab129163) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
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![OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] (ab129163)](https://www.abcam.com/ps/products/129/ab129163/Images/ab129163-143162-anti-nudt5-antibody-epr7735.jpg)
OI-RD Scanning - Anti-NUDT5 antibody [EPR7735] (ab129163) -
![Anti-NUDT5 antibody [EPR7735] (ab129163)](https://www.abcam.com/ps/products/129/ab129163/Images/ab129163-4-benefits-of-recombinant-antibodies.png)
Anti-NUDT5 antibody [EPR7735] (ab129163)
