Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 10, 2021

Anti-TUFM antibody [EPR12797(B)] (ab173300)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12797(B)] to TUFM
Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
Reacts with: Human

Product name

Anti-TUFM antibody [EPR12797(B)]
See all TUFM primary antibodies
Description

Rabbit monoclonal [EPR12797(B)] to TUFM
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TUFM aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P49411
Positive control
- HepG2, A431, HeLa, and Jurkat whole cell lysate (ab7899), Human breast and Human colon tissues, A431 cells and Jurkat cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12797(B)
Isotype

IgG
Research areas

Western blot - Anti-TUFM antibody [EPR12797(B)] (ab173300)

All lanes : Anti-TUFM antibody [EPR12797(B)] (ab173300) at 1/10000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 50 kDa
Immunoprecipitation - Anti-TUFM antibody [EPR12797(B)] (ab173300)

Immunoprecipitation. ab173300 at 1/10000 staining TUFM immunoprecipitated from HeLa lysate using ab173300 at 1/10.
Lane 2: 1X PBS (negative control).
Immunocytochemistry/ Immunofluorescence - Anti-TUFM antibody [EPR12797(B)] (ab173300)

Immunofluorescent analysis of A431 cells labeling TUFM with ab173300 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] (ab173300)

Immunohistochemical analysis of paraffin embedded Human breast tissue labeling TUFM with ab173300 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TUFM antibody [EPR12797(B)] (ab173300)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TUFM with ab173300 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-TUFM antibody [EPR12797(B)] (ab173300)

Flow Cytometrical analysis of permeabilized Jurkat cells with ab173300 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
Anti-TUFM antibody [EPR12797(B)] (ab173300)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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