Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-hnRNP G antibody [EPR16038] (ab190352)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16038] to hnRNP G
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-hnRNP G antibody [EPR16038]
    See all hnRNP G primary antibodies

  • Description

    Rabbit monoclonal [EPR16038] to hnRNP G

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 92% homology with human RbmxL1, 100% homology with mouse RbmxL1 and 85% homology with mouse RbmxL2. Cross-reactivity with these proteins have not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Jukat, K562, A431, mouse brain, rat brain, C6 and NIH/3T3 lysate. Human transitional cell carcinoma of bladder and mouse liver tissue. A431 and HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-hnRNP G antibody [EPR16038] (ab190352)
    Western blot - Anti-hnRNP G antibody [EPR16038] (ab190352)
    All lanes : Anti-hnRNP G antibody [EPR16038] (ab190352) at 1/1000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Jurkat lysate
    Lane 3 : K562 lysate
    Lane 4 : A431 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (ab190352)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (ab190352)

    Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling hnRNP G with ab190352 at 1/250 dilution. Counter stain Hematoxylin. Negative control also shown lower left.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (ab190352)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (ab190352)

    Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling hnRNP G with ab190352 at a 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution (Red). Negative controls and DAPI staining also shown.

  • Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (ab190352)
    Immunocytochemistry/ Immunofluorescence - Anti-hnRNP G antibody [EPR16038] (ab190352)

    Immunofluorescent analysis of 4% paraformaldehyde fixed A431 cells labeling hnRNP G with ab190352 at a 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution (Red). Negative controls and DAPI staining also shown.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (ab190352)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP G antibody [EPR16038] (ab190352)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling hnRNP G with ab190352 at 1/250 dilution. Counter stain Hematoxylin. Negative control also shown lower left.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-hnRNP G antibody [EPR16038] (ab190352)
    Western blot - Anti-hnRNP G antibody [EPR16038] (ab190352)
    All lanes : Anti-hnRNP G antibody [EPR16038] (ab190352) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : C6 lysate
    Lane 4 : NIH/3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

  • Anti-hnRNP G antibody [EPR16038] (ab190352)
    Anti-hnRNP G antibody [EPR16038] (ab190352)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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