Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 09, 2021

Anti-BACE2 antibody [EPR23339-221] (ab270458)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23339-221] to BACE2
Suitable for: IHC-Fr, WB, ICC/IF, IP, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-BACE2 antibody [EPR23339-221]
See all BACE2 primary antibodies
Description

Rabbit monoclonal [EPR23339-221] to BACE2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse Rat
IP	Mouse
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, A549, C6, Beta-TC-6 and Neuro 2a cell lysate. IHC-P: Mouse and rat pancreas tissue. IHC-fr: Mouse pancreas. ICC/IF: Beta-TC-6 cells. IP: Neuro-2a whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23339-221
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling BACE2 with ab270458 at 1/100 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling BACE2 with ab270458 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse pancreatic islet (PMID: 21907142). The section was incubated with ab270458 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)

All lanes : Anti-BACE2 antibody [EPR23339-221] (ab270458) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 3 : C6 (rat glial tumor glial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 56 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 136 secs.
Immunoprecipitation - Anti-BACE2 antibody [EPR23339-221] (ab270458)

BACE2 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab270458 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270458 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Neuro-2a whole cell lysate.

Lane 2: ab270458 IP in Neuro-2a whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270458 in Neuro-2a whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 mins.
Immunocytochemistry/ Immunofluorescence - Anti-BACE2 antibody [EPR23339-221] (ab270458)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 cells labeling BACE2 with ab270458 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic and membranous staining in Beta-TC-6 cell line. 100% methanol fixation is recommended. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BACE2 antibody [EPR23339-221] (ab270458)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling BACE2 with ab270458 at 1/100 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat pancreatic islet (PMID: 21907142). The section was incubated with ab270458 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-BACE2 antibody [EPR23339-221] (ab270458)

All lanes : Anti-BACE2 antibody [EPR23339-221] (ab270458) at 1/1000 dilution

Lane 1 : Beta-TC-6 (mouse pancreas insulinoma beta cell), whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 56 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 secs.
Anti-BACE2 antibody [EPR23339-221] (ab270458)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com