Anti-BACE2 antibody [1/9] (ab245679)
![Anti-BACE2 antibody [1/9] (ab245679)](https://www.abcam.com/ps/products/245/ab245679/Images/ab245679-336917-2456792.jpg "Anti-BACE2 antibody [1/9] (ab245679)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [1/9] to BACE2
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-BACE2 antibody [1/9]
See all BACE2 primary antibodies -
Description
Mouse monoclonal [1/9] to BACE2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human BACE2 aa 244-333.
Database link: Q9Y5Z0 -
Positive control
- ICC/IF: U-251 MG cells. WB: HeLa and U-251 MG cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1/9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-BACE2 antibody [1/9] (ab245679)
PFA fixed, 0.15% Triton permeabilized U-251 MG (Human brain glioma cell line) cells labeling BACE2 using ab245679 at 10 µg/ml for 1 hour (green) followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue0.
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![Western blot - Anti-BACE2 antibody [1/9] (ab245679)](https://www.abcam.com/ps/products/245/ab245679/Images/ab245679-336916-2456791.jpg)
Western blot - Anti-BACE2 antibody [1/9] (ab245679)All lanes : Anti-BACE2 antibody [1/9] (ab245679) at 1 µg/ml (incubated for 1 hr)
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate (protein in RIPA buffer)
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate (protein in RIPA buffer)
Lysates/proteins at 35 µg per lane.10% SDS PAGE gel.
Detected by chemiluminescence.
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![Anti-BACE2 antibody [1/9] (ab245679)](https://www.abcam.com/ps/products/245/ab245679/Images/ab245679-3-benefits-of-recombinant-antibodies.png)
Anti-BACE2 antibody [1/9] (ab245679)
