Anti-Nicastrin antibody [EPR2575Y] - BSA and Azide free (ab247362)
![Anti-Nicastrin antibody [EPR2575Y] - BSA and Azide free (ab247362)](https://www.abcam.com/ps/products/247/ab247362/Images/ab247362-410249-anti-nicastrin-antibody-epr2575y-western-blot-human-knockout-lysate.jpg "Anti-Nicastrin antibody [EPR2575Y] - BSA and Azide free (ab247362)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2575Y] to Nicastrin - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Nicastrin antibody [EPR2575Y] - BSA and Azide free
See all Nicastrin primary antibodies -
Description
Rabbit monoclonal [EPR2575Y] to Nicastrin - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, HeLa, Jurkat and HL-60 cell lysates.
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General notes
Ab247362 is the carrier-free version of ab68145. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247362 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR2575Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Nicastrin antibody [EPR2575Y] - BSA and Azide free (ab247362)All lanes : Anti-Nicastrin antibody [EPR2575Y] (ab68145) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : NCSTN knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HL-60 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 100-125 kDa why is the actual band size different from the predicted?This data was developed using ab68145, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - ab68145 observed at 100-125 kDa. Red - loading control ab8245 observed at 36 kDa.
ab68145 Anti-Nicastrin antibody [EPR2575Y] was shown to specifically react with Nicastrin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266602 (knockout cell lysate ab258061) was used. Wild-type and Nicastrin knockout samples were subjected to SDS-PAGE. ab68145 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Nicastrin antibody [EPR2575Y] - BSA and Azide free (ab247362)](https://www.abcam.com/ps/products/247/ab247362/Images/ab247362-399258-anti-nicastrin-antibody-epr2575y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Nicastrin antibody [EPR2575Y] - BSA and Azide free (ab247362)All lanes : Anti-Nicastrin antibody [EPR2575Y] (ab68145) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HL-60 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 78 kDa
Additional bands at: 100-120 kDa (possible isoform), 130-160 kDa (possible isoform)This data was developed using ab68145, the same antibody clone in a different buffer formulation.
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![Anti-Nicastrin antibody [EPR2575Y] - BSA and Azide free (ab247362)](https://www.abcam.com/ps/products/247/ab247362/Images/ab247362-3-benefits-of-recombinant-antibodies.png)
Anti-Nicastrin antibody [EPR2575Y] - BSA and Azide free (ab247362)
