Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitor Assay Kit (ab273304)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
Overview
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Product name
Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitor Assay Kit
See all Indoleamine 2, 3-dioxygenase kits -
Detection method
Fluorescent -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Indoleamine 2,3-Dioxygenase 1 (IDO1) Inhibitor Assay Kit (ab273304) enables rapid screening of test compounds for modulation of IDO1 activity.
Unlike conventional colorimetric IDO1 assays based upon UV absorbance, the assay uses a fluorogenic developer that selectively reacts with NFK to produce a highly fluorescent product detectable in the visible range (Ex/Em = 402/488 nm).
This ensures a high signal-to-background ratio and little interference due to short wavelength absorbance by test compounds. The assay is highly sensitive, has a simple no-wash protocol and is high-throughput adaptable. The kit contains a complete set of reagents sufficient for performing 100 reactions in a 96-well plate format.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Antioxidant Mix (100X) (Lyophilized) 1 vial Fluorogenic Developer Solution 1 x 5ml IDO1 Assay Buffer 1 x 50ml IDO1 Inhibitor (IDO5L) (Lyophilized) 1 vial IDO1 Substrate (L-Tryptophan)(Lyophilized) 1 vial Microplate Sealing Film 1 unit Recombinant Human IDO1 (Lyophilized) 1 vial -
Research areas
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Function
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. -
Pathway
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the indoleamine 2,3-dioxygenase family. - Information by UniProt
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Alternative names
- 3-dioxygenase
- I23O1_HUMAN
- IDO
see all
Images
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Example dose response curve
Example Data
Dose-response curve for IDO1 inhibition by the included selective IDO1 Inhibitor IDO5L. The IC50 value (24.2 ± 1.3 nM) was derived by 4-parameter logistic curve fitting with each point representing the mean ± EM of at least 3 replicates. All assays were performed according to the kit protocol.
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Measurement of IDO1 inhibition in presence and absence of 1 µM of IDO5LExample Data
Measurement of IDO1 inhibition in presence and absence of 1 μM of IDO5L (a potent, highly selective competitive IDO1 inhibitor) and 100 μM of norharmane (a natural product that acts as a weak IDO1 inhibitor). The no inhibitor reaction contained assay buffer with 0.1% DMSO (v/v) as a solvent control.